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- W76885227 abstract "Alkaline sucrose density gradient (ASDG) centrifugation is probably an only method to detect elongation of pulse-labeled replication products in cells. If the cells are pulse-labeled after being exposed to some DNA-damaging agents, their post-replication repair can be measured by ASDG technique. With non-damaged cells, normal replication in replicon size can be observed, too. In addition, the method is also applicable to measure single strand breaks. We have modified this classical method to reproducibly detect very long single-stranded DNA at the megabase level. Here, the protocols are optimized to DT40 cells." @default.
- W76885227 created "2016-06-24" @default.
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- W76885227 date "2006-01-01" @default.
- W76885227 modified "2023-09-26" @default.
- W76885227 title "Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation" @default.
- W76885227 doi "https://doi.org/10.1007/978-1-4020-4896-8_42" @default.
- W76885227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17623937" @default.
- W76885227 hasPublicationYear "2006" @default.
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