Matches in SemOpenAlex for { <https://semopenalex.org/work/W769798952> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W769798952 endingPage "104" @default.
- W769798952 startingPage "97" @default.
- W769798952 abstract "Footrot is an important infectious disease of small ruminants leading to severe economical losses. The aim of the present study was to determine isolation and identification rates of Dichelobacter nodosus and Fusobacterium necrophorum in the culture techniques and reveal the specificity and sensitivity of the culture technique based on the polymerase chain reaction (PCR) method in sheep with footrot. Dry swabs and swabs with Amies medium from 83 sheep were subjected to PCR and culture analyses. In dry swabs, 4 samples were positive for F. necrophorum and all were negative for D. nodosus . Colonies in Eugon and Fusobacterium selective agars from swabs with Amies medium were evaluated. Polymerase chain reaction analysis was conducted on macroscopically and microscopically unidentified samples. The positivity rate was 55.4% for D. nodosus and 69.8% for F. necrophorum in cultures from Fusobacterium selective agars. The positivity rate for D. nodosus in Fusobacterium selective agars was higher than that in Eugon agar. Performing PCR and culture methods increased positivity as compared to performing them alone. In comparison with the PCR method, culturing in Fusobacterium selective agars had moderate sensitivity and low specificity for D. nodosus (71.7 and 28.7%) and F. necrophorum (61.3 and 80.0%), respectively. In conclusion, Fusobacterium selective agar (without antibiotics) for isolation and identification of D. nodosus is superior to Eugon agar. Fusobacterium necrophorum should also be considered as a provoking agent for footrot in small ruminants. The PCR method on culture increases elucidation of definitive aetiology." @default.
- W769798952 created "2016-06-24" @default.
- W769798952 creator A5001474270 @default.
- W769798952 creator A5008975101 @default.
- W769798952 creator A5035050885 @default.
- W769798952 creator A5056652168 @default.
- W769798952 creator A5074041976 @default.
- W769798952 creator A5077437113 @default.
- W769798952 creator A5085996274 @default.
- W769798952 date "2015-01-01" @default.
- W769798952 modified "2023-10-03" @default.
- W769798952 title "Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot" @default.
- W769798952 cites W1979467129 @default.
- W769798952 cites W1980973411 @default.
- W769798952 cites W1984542309 @default.
- W769798952 cites W2000510425 @default.
- W769798952 cites W2007140389 @default.
- W769798952 cites W2027425312 @default.
- W769798952 cites W2046697589 @default.
- W769798952 cites W2060839927 @default.
- W769798952 cites W2067055295 @default.
- W769798952 cites W2069128438 @default.
- W769798952 cites W2084081589 @default.
- W769798952 cites W2094190392 @default.
- W769798952 cites W2094786907 @default.
- W769798952 cites W2118896108 @default.
- W769798952 cites W2154830498 @default.
- W769798952 cites W2162804919 @default.
- W769798952 cites W2164848254 @default.
- W769798952 cites W2058064805 @default.
- W769798952 doi "https://doi.org/10.2754/avb201584020097" @default.
- W769798952 hasPublicationYear "2015" @default.
- W769798952 type Work @default.
- W769798952 sameAs 769798952 @default.
- W769798952 citedByCount "2" @default.
- W769798952 countsByYear W7697989522017 @default.
- W769798952 countsByYear W7697989522021 @default.
- W769798952 crossrefType "journal-article" @default.
- W769798952 hasAuthorship W769798952A5001474270 @default.
- W769798952 hasAuthorship W769798952A5008975101 @default.
- W769798952 hasAuthorship W769798952A5035050885 @default.
- W769798952 hasAuthorship W769798952A5056652168 @default.
- W769798952 hasAuthorship W769798952A5074041976 @default.
- W769798952 hasAuthorship W769798952A5077437113 @default.
- W769798952 hasAuthorship W769798952A5085996274 @default.
- W769798952 hasBestOaLocation W7697989521 @default.
- W769798952 hasConcept C104317684 @default.
- W769798952 hasConcept C189539697 @default.
- W769798952 hasConcept C2775941552 @default.
- W769798952 hasConcept C2778168646 @default.
- W769798952 hasConcept C2778660310 @default.
- W769798952 hasConcept C2779141898 @default.
- W769798952 hasConcept C2780960513 @default.
- W769798952 hasConcept C49105822 @default.
- W769798952 hasConcept C523546767 @default.
- W769798952 hasConcept C54355233 @default.
- W769798952 hasConcept C55493867 @default.
- W769798952 hasConcept C62643968 @default.
- W769798952 hasConcept C86803240 @default.
- W769798952 hasConcept C89423630 @default.
- W769798952 hasConceptScore W769798952C104317684 @default.
- W769798952 hasConceptScore W769798952C189539697 @default.
- W769798952 hasConceptScore W769798952C2775941552 @default.
- W769798952 hasConceptScore W769798952C2778168646 @default.
- W769798952 hasConceptScore W769798952C2778660310 @default.
- W769798952 hasConceptScore W769798952C2779141898 @default.
- W769798952 hasConceptScore W769798952C2780960513 @default.
- W769798952 hasConceptScore W769798952C49105822 @default.
- W769798952 hasConceptScore W769798952C523546767 @default.
- W769798952 hasConceptScore W769798952C54355233 @default.
- W769798952 hasConceptScore W769798952C55493867 @default.
- W769798952 hasConceptScore W769798952C62643968 @default.
- W769798952 hasConceptScore W769798952C86803240 @default.
- W769798952 hasConceptScore W769798952C89423630 @default.
- W769798952 hasIssue "2" @default.
- W769798952 hasLocation W7697989521 @default.
- W769798952 hasLocation W7697989522 @default.
- W769798952 hasOpenAccess W769798952 @default.
- W769798952 hasPrimaryLocation W7697989521 @default.
- W769798952 hasRelatedWork W1563035761 @default.
- W769798952 hasRelatedWork W2018523446 @default.
- W769798952 hasRelatedWork W2037212963 @default.
- W769798952 hasRelatedWork W2042484813 @default.
- W769798952 hasRelatedWork W2046982600 @default.
- W769798952 hasRelatedWork W2052439014 @default.
- W769798952 hasRelatedWork W2114355664 @default.
- W769798952 hasRelatedWork W2184969992 @default.
- W769798952 hasRelatedWork W4243997516 @default.
- W769798952 hasRelatedWork W769798952 @default.
- W769798952 hasVolume "84" @default.
- W769798952 isParatext "false" @default.
- W769798952 isRetracted "false" @default.
- W769798952 magId "769798952" @default.
- W769798952 workType "article" @default.