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- W770223732 abstract "In this chapter, some specialized applications are described in which computers are essential for manipulating and enhancing images obtained by using a transmission electron microscope, particularly to understand the tertiary and quaternary structures of biological macromolecules and their complexes. The chapter must be read with some knowledge of the basic principles of transmission electron microscopy (TEM) of biological material. The computational techniques for analyzing and reconstructing macromolecular structures from TEM images are reviewed in monographs. Partial results of single-particle analyses of both frozen-hydrated and negatively stained preparations are illustrated in the chapter. S-layers, the proteinaceous surface arrays found on some gram-positive and gram-negative eubacteria and many archaea, are naturally occurring 2-D crystals that are amenable to image analysis by electron crystallography. TEM, particularly cryoTEM, has become a powerful tool for analyzing the tertiary and quaternary conformations of biological macromolecules and their complexes. Computational image analysis and reconstruction are integral tools in this process. The chapter has illustrated two approaches to manipulating and processing macromolecular TEM images that one would commonly find in the structural and molecular microbiological literature." @default.
- W770223732 created "2016-06-24" @default.
- W770223732 creator A5074237429 @default.
- W770223732 date "2014-04-30" @default.
- W770223732 modified "2023-09-24" @default.
- W770223732 title "Computational Image Analysis and Reconstruction from Transmission Electron Micrographs" @default.
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- W770223732 doi "https://doi.org/10.1128/9781555817497.ch5" @default.
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