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- W77040521 abstract "Hybridoma cells secrete a factor (referred to in the following as ITI: inhibitor of radioactive thymidine incorporation) which inhibits incorporation of radioactive thymidine into cells 1. Of other cells tested, only myeloma cells were found to secrete ITI 2. It has been suggested that the factor prevents growth of hybridoma cells to high cell densities and thus limits the amount of monoclonal antibodies that may be obtained from batch cultures of hybridoma cells 1,2. In this study, we have purified ITI to homogeneity and through structural determination by nuclear magnetic resonance (NMR) spectroscopy we have identified it as thymidine. The results indicate that hybridoma and (some) myeloma cells secrete relative large amounts of thymidine to their surroundings." @default.
- W77040521 created "2016-06-24" @default.
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- W77040521 date "2006-03-01" @default.
- W77040521 modified "2023-09-26" @default.
- W77040521 title "Secretion of Thymidine by Hybridoma Cells" @default.
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- W77040521 doi "https://doi.org/10.1007/0-306-46843-3_58" @default.
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