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• W77069942 abstract "Fluorescence has played a vital role in the development of polymerase chain reaction (PCR)-based DNA amplification. In qualitative PCR, an end point reaction, the amplified DNA, is visualized using DNA intercalating fluorescent dyes. Creative uses of nucleotide probes with fluorescent tags have been developed for real-time quantitative PCR. These probes take advantage of the behavior and properties of fluorophores. There are advantages and disadvantages to various probe types as well as design considerations. Attention to these issues will help in the development of robust and accurate DNA quantification using real-time PCR. Open image in new window" @default.
• W77069942 created "2016-06-24" @default.
• W77069942 creator A5023492006 @default.
• W77069942 creator A5050113094 @default.
• W77069942 date "2008-03-12" @default.
• W77069942 modified "2023-10-06" @default.
• W77069942 title "Quantitative Real-Time PCR: Fluorescent Probe Options and Issues" @default.
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• W77069942 doi "https://doi.org/10.1007/4243_2008_046" @default.
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