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- W77118772 abstract "Abstract : An enzyme-limited immunosorbent-assays (ELISA) with electrochemical detection was developed for the seafood toxin okadaic acid (diarrhetic shellfish poison). At low ppb concentrations, competitive heterogeneous immunoassays were performed in which soluble sample antigen (50 pl) competed with immobilized antigen for soluble antibody (50 pl). Excess sample and antibody were removed by washing and a secondary antibody labeled with alkaline phosphatase was added. The enzyme label hydrolyzed phenyl phosphate to yield phenol which was oxidized at +870 mV vs. Ag/AgCl. The dynamic response was inversely proportional to soluble antigen concentration.... Electrochemical immunoassay, seafood toxins, okadaic acid, saxitoxin." @default.
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- W77118772 date "1990-03-01" @default.
- W77118772 modified "2023-09-24" @default.
- W77118772 title "Electrode Probes for the Rapid Assay of Seafood Toxicants. Phase 1" @default.
- W77118772 hasPublicationYear "1990" @default.
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