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• W77147165 abstract "Metabolism encompasses the biochemical basis of life, but even in well studied model organisms our knowledge of metabolism is incomplete (Downs, 2006). Sugars represent a major source of carbon supporting heterotrophic growth in Archaea and are oxidized via a conserved set of central metabolic pathways, including modified versions of the Entner-Doudoroff (ED) pathway. Sulfolobus solfataricus is a hyperthermophilic archaeon from the kingdom crenarchaeota. It is a strict aerobe that grows optimally at 80-85C, pH 2-4, and utilises numerous carbon sources including the four most-commonly occurring sugars in nature, D-glucose, D-galactose, D-xylose and L-arabinose. In S. solfataricus, glucose and galactose are metabolised through the non-phosphorylative ED pathway in which a single set of enzymes are utilised for the catabolism of both sugars, leading to the proposal of a metabolically promiscuous pathway. The first section of this pathway includes three enzymes, glucose dehydrogenase (GDH), gluconate dehydratase (GD) and 2-keto-3-deoxy-gluconate (KDG) aldolase, two of which, GDH and KDG aldolase, are found to have activity with pentose as well as hexose sugars, although the third enzyme, GD, is hexose specific. KDG-aldolase cleaves hexose substrates into pyruvate and glyceraldehyde, and pentoses into pyruvate and the C-2 compound glycoaldehyde. Glyceraldehyde is converted into a second molecule of pyruvate, but the fate of glycoaldehyde is currently unknown. In this thesis, the complete pathway for D-xylose and L-arabinose catabolism is elucidated. Through the detection of enzyme activities in the native organism and subsequent characterisation of recombinantly produced enzymes, the results presented show that a separate C5 dehydratase exists, completing the first part of a non-phosphorylative C5 pathway, and that the subsequent enzymes required for glycolaldehyde metabolism (glycolaldehyde oxidoreductase, glycolate oxidase and malate synthase) are present and active during growth on C5 sugars. In addition to the metabolic investigations, rational design and site-directed mutagenesis were used to explore the structural basis of substrate promiscuity of glucose dehydrogenase; in doing so, the enzyme was successfully engineered to oxidise the sugar D-ribose while retaining its activity with its natural substrates. These same techniques have been used to explore the relationship between structure, stability and catalytic activity of the S. solfataricus malate synthase, and the data have been fitted to a quantitative kinetic model that explores how temperature influences the activity of an enzyme." @default.
• W77147165 created "2016-06-24" @default.
• W77147165 creator A5055676322 @default.
• W77147165 date "2010-01-01" @default.
• W77147165 modified "2023-09-25" @default.
• W77147165 title "The Enzymology of Sugar Metabolism in Sulfolobus solfataricus" @default.
• W77147165 cites W125405689 @default.
• W77147165 cites W143128980 @default.
• W77147165 cites W1480396224 @default.
• W77147165 cites W1516366759 @default.
• W77147165 cites W1520173007 @default.
• W77147165 cites W1526690553 @default.
• W77147165 cites W1531341650 @default.
• W77147165 cites W1533284863 @default.
• W77147165 cites W1575215071 @default.
• W77147165 cites W1584570642 @default.
• W77147165 cites W1590712764 @default.
• W77147165 cites W1657410952 @default.
• W77147165 cites W1687960050 @default.
• W77147165 cites W1748668484 @default.
• W77147165 cites W1814486098 @default.
• W77147165 cites W1901024895 @default.
• W77147165 cites W1921593426 @default.
• W77147165 cites W1961858939 @default.
• W77147165 cites W1964196548 @default.
• W77147165 cites W1965082449 @default.
• W77147165 cites W1967118468 @default.
• W77147165 cites W1967231628 @default.
• W77147165 cites W1968676324 @default.
• W77147165 cites W1969021085 @default.
• W77147165 cites W1969175129 @default.
• W77147165 cites W1970419053 @default.
• W77147165 cites W1970710213 @default.
• W77147165 cites W1971383763 @default.
• W77147165 cites W1972409069 @default.
• W77147165 cites W1974961023 @default.
• W77147165 cites W1976881268 @default.
• W77147165 cites W1977163896 @default.
• W77147165 cites W1977962237 @default.
• W77147165 cites W1978508532 @default.
• W77147165 cites W1979510496 @default.
• W77147165 cites W1980776051 @default.
• W77147165 cites W1980782731 @default.
• W77147165 cites W1981159620 @default.
• W77147165 cites W1984890497 @default.
• W77147165 cites W1986111972 @default.
• W77147165 cites W1989305888 @default.
• W77147165 cites W1990456200 @default.
• W77147165 cites W1991422330 @default.
• W77147165 cites W1992260476 @default.
• W77147165 cites W1995204321 @default.
• W77147165 cites W1995683139 @default.
• W77147165 cites W1995706284 @default.
• W77147165 cites W1996320023 @default.
• W77147165 cites W1996822335 @default.
• W77147165 cites W1997146378 @default.
• W77147165 cites W1998238795 @default.
• W77147165 cites W1998313482 @default.
• W77147165 cites W2003436519 @default.
• W77147165 cites W2003890287 @default.
• W77147165 cites W2005355917 @default.
• W77147165 cites W2005824211 @default.
• W77147165 cites W2006405683 @default.
• W77147165 cites W2007158632 @default.
• W77147165 cites W2007298287 @default.
• W77147165 cites W2008921808 @default.
• W77147165 cites W2012968714 @default.
• W77147165 cites W2015546264 @default.
• W77147165 cites W2015761686 @default.
• W77147165 cites W2016717383 @default.
• W77147165 cites W2016943354 @default.
• W77147165 cites W2017661263 @default.
• W77147165 cites W2018786644 @default.
• W77147165 cites W2021518677 @default.
• W77147165 cites W2022280363 @default.
• W77147165 cites W2024383780 @default.
• W77147165 cites W2027256956 @default.
• W77147165 cites W2027741507 @default.
• W77147165 cites W2028113659 @default.
• W77147165 cites W2028994826 @default.
• W77147165 cites W2030081928 @default.
• W77147165 cites W2035174751 @default.
• W77147165 cites W2035648603 @default.
• W77147165 cites W2038776520 @default.
• W77147165 cites W2039281572 @default.
• W77147165 cites W2042045910 @default.
• W77147165 cites W2042181696 @default.
• W77147165 cites W2044172420 @default.
• W77147165 cites W2046829935 @default.
• W77147165 cites W2047804361 @default.
• W77147165 cites W2047849624 @default.
• W77147165 cites W2051419400 @default.
• W77147165 cites W2053159677 @default.
• W77147165 cites W2054618890 @default.
• W77147165 cites W2055204850 @default.
• W77147165 cites W2055336370 @default.
• W77147165 cites W2056679161 @default.
• W77147165 cites W2057427542 @default.
• W77147165 cites W2057596435 @default.
• W77147165 cites W2058189144 @default.

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