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- W771565235 abstract "Bacteriophage T4 DNA containing cytosine has been obtained from cells infected with phage mutants in genes $6, denA, denB, and ale. This DNA can be cut with a number of restriction endonucleases. How ever, DNA containing nonglucosylated hydroxymethylcytosine is either refractory to restriction or the patterns of restriction are partial and irreproducible. Fragments obtained by restriction of cytosine containing DNA with Pstl have been cloned using the plasmid vector pBR322. Plasmids containing ligated fragments were transformed into thymidylate synthe tase negative (td**) host cells. Two clones were isolated with the ability to grow in the absence of added thymine. Both isolates express thymidylate synthetase activity as determined by enzymatic assays. A class of deletion mutants in T4 has been reported in the td-dfr region by Homyk and Weil. Our data show that these mutants cross react with antisera prepared against td and dfr, revert on nonpermissive bacterial strains, and produce normal levels of dfr upon removal of a second independent deletion. This evidence is in direct contradiction to the original work which reported that these deletions spanned the entire td-frd region." @default.
- W771565235 created "2016-06-24" @default.
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- W771565235 date "1979-01-01" @default.
- W771565235 modified "2023-09-28" @default.
- W771565235 title "The molecular cloning of bacteriophage T4: the identification and expression of a clone coding for bacteriophage thymidylate synthetase" @default.
- W771565235 hasPublicationYear "1979" @default.
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