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- W772805180 abstract "Milk-borne factors delivered to nursing offspring via a lactocrine mechanism affect development of somatic tissues, including the uterus. Gene expression events associated with porcine endometrial maturation between birth (postnatal day = PND 0) and PND 2 can determine and may define the uterine developmental program. Lactocrine-sensitive elements of the neonatal porcine uterine transcriptome have not been defined during this period. Therefore, the objective here was to determine effects of imposition of the lactocrine-null condition for 48 h from birth on the porcine uterine transcriptome at PND 2 using RNA sequencing (RNAseq). Gilts (n = 4/group) were assigned at birth to be: (1) nursed ad libitum, or (2) gavage-fed (30 ml/kg BW/2h) commercial porcine milk-replacer for 48 h. Uteri were obtained at 50 h. Total uterine RNA was extracted and both concentration and integrity were determined. For each uterus, 500 ng of RNA was used for cDNA library preparation. Libraries were sequenced (paired end) at > 90 million reads per sample. After demultiplexing, raw reads were mapped to the latest pig Sscrofa10.2 build using Avadis NGS software. Validation compared RNAseq results to those obtained for targeted genes using quantitative RT-PCR. Gene enrichment and functional analyses were conducted. On PND 2, 896 genes were differentially expressed (P < 0.05, corrected for false discovery rate) in nursed as compared to milk replacer-fed gilts. Gene enrichment and functional analyses revealed alterations in multiple biological processes and pathways including estrogen receptor-alpha and hedgehog signaling pathways, and the plasminogen activating network. Results can be used to refine and focus hypotheses related to identification of cellular, molecular, and lactocrine mechanisms regulating neonatal uterine and endometrial development." @default.
- W772805180 created "2016-06-24" @default.
- W772805180 creator A5010141887 @default.
- W772805180 date "2014-01-01" @default.
- W772805180 modified "2023-09-23" @default.
- W772805180 title "MILK-BORNE BIOACTIVE FACTORS: EFFECTS ON NEONATAL PORCINE REPRODUCTIVE TISSUES" @default.
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