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- W774241853 abstract "The wheat-rye translocation lines have been agriculturally developed for the crop resistant to powdery mildew, leaf rust, Hessian fly, barley yellow dwarf virus, and drought stress. Chaupon rye contains 2RL chromatin to harbor resistance genes for powdery mildew and leaf rust. In order to identify 2RL chromosome-derived specific proteins, we compared the proteome of ‘Coker797’ (non-2RL) with those of ‘Hamlet’ (2RL) and near-isogenic line (NIL) carrying 2RL by 2D-gel electrophoresis and MALDI/ESI-MS. In the leaf proteome, 24 protein spots were clearly increased in 2RL-carrying lines compared to non-2RL line. The specific proteins of 2RL-lines included heat shock protein 70, chaperon protein DnaK, malate dehydrogenase I, and triosephosphate isomerase, which were confirmed in the EST database of NILs. In the root proteome, three protein spots were identified as putative peroxidase, cytoplasmic aldolase, and oxo-phytodienoic acid reductase. These results suggest that defense mechanism-related proteins and enhanced catabolic enzymes play roles of acquiring the resistance to biotic and abiotic stress in wheat-rye translocation lines." @default.
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- W774241853 date "2008-01-01" @default.
- W774241853 modified "2023-09-27" @default.
- W774241853 title "Proteins identification of wheat-rye translocation lines by MALDI-TOF-TOF mass spectrometry and ESI-QTOF/MS" @default.
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