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- W776066686 abstract "The rice centromeric clone 6730.t11, located in the kinetochore region of the rice chromosome 8 centromere, was mapped to the centromeric regions of wheat group-7 chromosomes by Southern hybridization. PCR amplification with an RT-PCR (Reverse Transcription-PCR) primer of 6730.t11 was conducted on genomic DNA isolated from Triticeae species, including T. urartu, T. monococcum subsp. monococcum, T. monococcum subsp. aegilopoides, Ae. speltoides, Ae. tauschii, barley, rye, and H. villosa, the rice cultivars (O. sativa subsp. Japonica) ‘Nipponbare’ and (O. sativa subsp. Indica) ‘IRBB7’, maize, soybean, tomato, and Arabidopsis. A 211-bp sequence was amplified from Nipponbare, which showed 100% identity to the sequenced rice genomic DNA covered by the 6730.t11 RT-PCR primer. Of eight plasmid clones of PCR products sequenced from IRBB7, six had the same 211-bp sequence as found in Nipponbare. Two clones had a 202-bp sequence that shared 100 percent and 87 percent similarity in the first 38 and the last 72 nucleotides, respectively, with the 211-bp sequence amplified from Nipponbare. Surprisingly, the 202-bp sequence amplified from IRBB7 was found in all monocot and dicot species used in this study except Nipponbare. Sequence similarity ranged from 99% to 100% when compared to the 202-bp sequence in IRBB7. A PCR-amplified fragment from genomic DNA of Chinese Spring (CS) wheat using an RT-PCR primer of the clone 6730.t11 was mapped to the same region as clone 6730.t11 in wheat. The RT-PCR results from CS cDNA with primers of 6730.t11 indicated that the rice centromeric gene was expressed in wheat leaf tissue. Our data demonstrate strong selection pressure for the conservation of this DNA element in the kinetochore region of plants, although its functional role remains to be established." @default.
- W776066686 created "2016-06-24" @default.
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- W776066686 date "2008-01-01" @default.
- W776066686 modified "2023-09-24" @default.
- W776066686 title "A rice centromeric sequence is conserved between wheat and rice; as well as between monocots and dicots" @default.
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