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- W77808897 abstract "Crown rot(CR)of wheat, caused by Fusarium pseudograminearum (Fp) is an important disease globally, resulting in reduced yield as well as contamination with trichothecene mycotoxins, such as deoxynivalenol(DON).This hostnpathogen interaction has not been studied previously at the molecular level.To understand the host response to challenge by Fp, we have used the Affymetrix GeneChip Wheat Genome Array to examine gene expression changes in the wheat stem base following inoculation with Fp macroconidia. Induced genes included mainly those with defensive functions, such as genes encoding antinmicrobial proteins, as well as oxidative stressnrelated proteins, signallingnrelated proteins, and those involved in both primary and secondary metabolism.Expression of selected Fpninduced genes was confirmed using realntime quantitative PCR on an inoculation timencourse of two wheat cultivars, Kennedy and Sunco.Interestingly, several genes were induced earlier, and to higher levels, in the partially CR resistant cultivar Sunco than susceptible Kennedy.Many Fpninduced genes were also induced by methyljasmonate, and treatment with this compound prior to inoculation delayed the development of CR symptoms suggesting thatjasmonates may be involved in activating defences during crown rot. In this study, the response of wheat tissue to DON has also been investigated.Infusion of wheat leaves with DON induced hydrogen peroxide production within 6h, followed by cell death within 24h that was accompanied by DNA laddering, a hallmark of programmed cell death.Most of the genes identified here as being induced by Fp were also induced by DON in a concentrationndependent manner.Contreatment with DON and the antioxidant ascorbic acid reduced these responses suggesting their induction may be at least partially mediated by reactive oxygen species(ROS), commonly known to be signalling molecules in plants. Taken together, results are consistent with a model where DON production during infection of wheat induces ROS, which on one hand may stimulate programmed host cell death assisting necrotrophic fungal growth, but on the other hand the ROS may contribute to the induction of antinmicrobial host defences.This study is the first comprehensive analysis of wheat responses induced during CR and provides new insights into the host processes potentially involved in wheat defences against Fusarium pathogens." @default.
- W77808897 created "2016-06-24" @default.
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- W77808897 date "2008-01-01" @default.
- W77808897 modified "2023-09-26" @default.
- W77808897 title "Wheat defence responses induced by the fungal pathogen Fusarium pseudograminearum" @default.
- W77808897 hasPublicationYear "2008" @default.
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