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• W77889823 abstract "LECT2 is a chemotactic protein to neutrophils. A recombinant six-histidine-tagged human LECT2, (His)(6)-LECT2, was expressed in E. coli using a pET21a(+) vector. The (His)(6)-LECT2 was purified from the soluble fraction in E. coli as a single band in sodium dodesyl sulfate/polyacrylamide gel electrophoresis using three steps of column chromatography with Ni(2+)-charged nitrilo-triacetic acid (Ni-NTA) agarose, DEAE-Sepharose, and CM-Sepharose. The purified (His)(6)-LECT2 was yielded with 96 µg from the soluble fraction of 1,500 ml culture of E. coli. The circular dichroism spectrum of (His)(6)-LECT2 showed the folded structure, which is rich in β-sheet structure and rare in α-helix." @default.
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• W77889823 doi "https://doi.org/10.1023/a:1007976103088" @default.
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