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- W778963997 abstract "Dissimilatory iron reduction is one of the earliest, perhaps the first form of an anoxic respiratory system on earth. Microorganisms harboring this system couple their growth to the transfer of electrons from various electron donors to the terminal electron acceptor Fe(III) via terminal ferric iron reductases. Its function in bioremediation and biocorrosion processes and in the generation of electricity from bioorganic material indicates a great potential for innovations in the future. This special form of metal respiration is distributed among phylogenetically distinct prokaryotes living in diverse marine and terrestrial habitats. Some proteins taking part in the electron transport to the terminal Fe(III) have already been identified and a few models for dissimilatory Fe(III) reduction of Shewanella and Geobacter species have been described. In this study, the dissimilatory Fe(IIII) respiration was analyzed, using a metagenomic approach that is independent of cultivation. In order to assess the diversity of genes encoding dissimilatory Fe(III) reductases, metagenomic gene libraries were prepared from environmental DNAs that have been isolated from grassland, river sediment, thermal spring sediment or agricultural soil samples. Subsequently, the constructed metagenomic libraries were screened for the presence of genes encoding dissimilatory Fe(III) reductases by a function-based assay. The screening of approximately 4 million clones resulted in the identification of six positive clones. The recombinant plasmids recovered from these clones enabled the Escherichia coli host to perform dissimilatory Fe(III) reduction under anaerobic conditions. Sequence analyses of the inserts of the plasmids revealed a phylogenetic affiliation of the cloned metagenomic DNA-fragments with Actinobacteria, Firmicutes and the α, β, and γ subgroups of the Proteobacteria. The products deduced from the identified genes showed significant sequence similarities to oxidoreductases, cytochrome C, and to proteins whose involvement in electron transport processes is unclear. In addition, iron-reducing bacteria were isolated from the studied environments via Iron-Minimal Media and were pyhlogenetically and bio-chemically characterised." @default.
- W778963997 created "2016-06-24" @default.
- W778963997 creator A5001001107 @default.
- W778963997 date "2022-02-20" @default.
- W778963997 modified "2023-09-26" @default.
- W778963997 title "Identifikation von Genen und Mikroorganismen, die an der dissimilatorischen Fe(III)-Reduktion beteiligt sind" @default.
- W778963997 doi "https://doi.org/10.53846/goediss-1359" @default.
- W778963997 hasPublicationYear "2022" @default.
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