Matches in SemOpenAlex for { <https://semopenalex.org/work/W77997651> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W77997651 abstract "To obtain a significant therapeutic effect transplanted genetically modified cells should have an enhanced ability to survive and active expression of the therapeutic gene. In this paper, by using immunofluorescent staining we investigated the functional activity of the gene-cell formulation designed to deliver a therapeutic gene into the area of regeneration. As a model we used transgenic SOD1G93A mice with amyotrophic lateral sclerosis phenotype which received xenotransplantation of human umbilical cord blood mononuclear cells, genetically modified with adenoviral expression vector encoding vascular endothelial growth factor (VEGF) and the reporter green fluorescent protein (EGFP). Results of the study allowed to establish not only the duration of survival of transplanted cells, but also the efficiency of expression of recombinant genes in genetically modified cells in vivo. Double immunofluorescent staining with antibodies against human nuclear antigen HNA and VEGF detected HNA+/VEGF+ cells in the terminal stage of disease 15 weeks after transplantation. These data suggest that genetically modified umbilical cord blood mononuclear cells, transplanted into SOD1-G93A transgenic mice, are able to penetrate the blood-brain barrier and migrate into the area of degeneration of nerve tissue and survive from the time of transplantation until the death of animals at the terminal stage of disease. At that time adenoviral expression vector encoding therapeutic gene is functionally active in transplanted cells, and secretory products of recombinant gene act on target cells by a paracrine mechanism." @default.
- W77997651 created "2016-06-24" @default.
- W77997651 creator A5003682953 @default.
- W77997651 creator A5008928332 @default.
- W77997651 creator A5016307871 @default.
- W77997651 creator A5017670787 @default.
- W77997651 creator A5031418888 @default.
- W77997651 creator A5042885259 @default.
- W77997651 creator A5045575672 @default.
- W77997651 creator A5049142177 @default.
- W77997651 creator A5051210510 @default.
- W77997651 creator A5059385682 @default.
- W77997651 creator A5066414193 @default.
- W77997651 creator A5069048701 @default.
- W77997651 creator A5070568257 @default.
- W77997651 creator A5071959037 @default.
- W77997651 creator A5073828136 @default.
- W77997651 creator A5091055929 @default.
- W77997651 creator A5091529063 @default.
- W77997651 date "2012-01-01" @default.
- W77997651 modified "2023-10-03" @default.
- W77997651 title "Анализ экспрессии рекомбинантного гена vegf генетически модифицированными мононуклеарными клетками пуповинной крови в эксперименте in vivo" @default.
- W77997651 hasPublicationYear "2012" @default.
- W77997651 type Work @default.
- W77997651 sameAs 77997651 @default.
- W77997651 citedByCount "0" @default.
- W77997651 crossrefType "journal-article" @default.
- W77997651 hasAuthorship W77997651A5003682953 @default.
- W77997651 hasAuthorship W77997651A5008928332 @default.
- W77997651 hasAuthorship W77997651A5016307871 @default.
- W77997651 hasAuthorship W77997651A5017670787 @default.
- W77997651 hasAuthorship W77997651A5031418888 @default.
- W77997651 hasAuthorship W77997651A5042885259 @default.
- W77997651 hasAuthorship W77997651A5045575672 @default.
- W77997651 hasAuthorship W77997651A5049142177 @default.
- W77997651 hasAuthorship W77997651A5051210510 @default.
- W77997651 hasAuthorship W77997651A5059385682 @default.
- W77997651 hasAuthorship W77997651A5066414193 @default.
- W77997651 hasAuthorship W77997651A5069048701 @default.
- W77997651 hasAuthorship W77997651A5070568257 @default.
- W77997651 hasAuthorship W77997651A5071959037 @default.
- W77997651 hasAuthorship W77997651A5073828136 @default.
- W77997651 hasAuthorship W77997651A5091055929 @default.
- W77997651 hasAuthorship W77997651A5091529063 @default.
- W77997651 hasConcept C104317684 @default.
- W77997651 hasConcept C111599444 @default.
- W77997651 hasConcept C126322002 @default.
- W77997651 hasConcept C137061746 @default.
- W77997651 hasConcept C142724271 @default.
- W77997651 hasConcept C150903083 @default.
- W77997651 hasConcept C153911025 @default.
- W77997651 hasConcept C202751555 @default.
- W77997651 hasConcept C203014093 @default.
- W77997651 hasConcept C207001950 @default.
- W77997651 hasConcept C26291073 @default.
- W77997651 hasConcept C2778442404 @default.
- W77997651 hasConcept C2911091166 @default.
- W77997651 hasConcept C32470452 @default.
- W77997651 hasConcept C40767141 @default.
- W77997651 hasConcept C502942594 @default.
- W77997651 hasConcept C55493867 @default.
- W77997651 hasConcept C71924100 @default.
- W77997651 hasConcept C86803240 @default.
- W77997651 hasConceptScore W77997651C104317684 @default.
- W77997651 hasConceptScore W77997651C111599444 @default.
- W77997651 hasConceptScore W77997651C126322002 @default.
- W77997651 hasConceptScore W77997651C137061746 @default.
- W77997651 hasConceptScore W77997651C142724271 @default.
- W77997651 hasConceptScore W77997651C150903083 @default.
- W77997651 hasConceptScore W77997651C153911025 @default.
- W77997651 hasConceptScore W77997651C202751555 @default.
- W77997651 hasConceptScore W77997651C203014093 @default.
- W77997651 hasConceptScore W77997651C207001950 @default.
- W77997651 hasConceptScore W77997651C26291073 @default.
- W77997651 hasConceptScore W77997651C2778442404 @default.
- W77997651 hasConceptScore W77997651C2911091166 @default.
- W77997651 hasConceptScore W77997651C32470452 @default.
- W77997651 hasConceptScore W77997651C40767141 @default.
- W77997651 hasConceptScore W77997651C502942594 @default.
- W77997651 hasConceptScore W77997651C55493867 @default.
- W77997651 hasConceptScore W77997651C71924100 @default.
- W77997651 hasConceptScore W77997651C86803240 @default.
- W77997651 hasIssue "3" @default.
- W77997651 hasLocation W779976511 @default.
- W77997651 hasOpenAccess W77997651 @default.
- W77997651 hasPrimaryLocation W779976511 @default.
- W77997651 hasVolume "7" @default.
- W77997651 isParatext "false" @default.
- W77997651 isRetracted "false" @default.
- W77997651 magId "77997651" @default.
- W77997651 workType "article" @default.