Matches in SemOpenAlex for { <https://semopenalex.org/work/W780562451> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W780562451 endingPage "311" @default.
- W780562451 startingPage "306" @default.
- W780562451 abstract "A new PCR method was developed to detect Pectobacterium carotovorum which is the causative agent of soft rot in Brassica pekinensis. A specific detection primer set based on Lytic murein transglycolase gene was designed and evaluated. Using ERB3_F (5'-TGC GAC ACC TCC TCA TCA CG-3') and ERB3_R (5'-CTT ATC ACG CTG TAA CCA GC-3') primers, 437 nucleotides long fragment was specifically amplified. The amplified products were observed in 52 out of 55 strains of P. carotovorum or Pectobacterium carotovorum subsp. carotovorum. On the other hand, no amplification was observed in 8 organisms including Chinese cabbage and potato. The optimal PCR condition for the ERB3_F/ERB3_R primer set was for annealing and 15 mM for . With serially diluted templates, the specific PCR sensitivitie limit was copies. Also, this method can be applied not only to DNA but also to field samples. This PCR method may be expected to be useful for specific detection of P. carotovorum." @default.
- W780562451 created "2016-06-24" @default.
- W780562451 creator A5004854316 @default.
- W780562451 creator A5022852733 @default.
- W780562451 creator A5027104860 @default.
- W780562451 creator A5058137473 @default.
- W780562451 creator A5084021009 @default.
- W780562451 date "2009-01-01" @default.
- W780562451 modified "2023-09-23" @default.
- W780562451 title "Development of a New PCR Method for Detection of Pectobacterium carotovorum" @default.
- W780562451 hasPublicationYear "2009" @default.
- W780562451 type Work @default.
- W780562451 sameAs 780562451 @default.
- W780562451 citedByCount "1" @default.
- W780562451 crossrefType "journal-article" @default.
- W780562451 hasAuthorship W780562451A5004854316 @default.
- W780562451 hasAuthorship W780562451A5022852733 @default.
- W780562451 hasAuthorship W780562451A5027104860 @default.
- W780562451 hasAuthorship W780562451A5058137473 @default.
- W780562451 hasAuthorship W780562451A5084021009 @default.
- W780562451 hasConcept C104317684 @default.
- W780562451 hasConcept C153911025 @default.
- W780562451 hasConcept C178790620 @default.
- W780562451 hasConcept C185592680 @default.
- W780562451 hasConcept C2776748224 @default.
- W780562451 hasConcept C2777563447 @default.
- W780562451 hasConcept C2780237616 @default.
- W780562451 hasConcept C49105822 @default.
- W780562451 hasConcept C54355233 @default.
- W780562451 hasConcept C86803240 @default.
- W780562451 hasConcept C89423630 @default.
- W780562451 hasConceptScore W780562451C104317684 @default.
- W780562451 hasConceptScore W780562451C153911025 @default.
- W780562451 hasConceptScore W780562451C178790620 @default.
- W780562451 hasConceptScore W780562451C185592680 @default.
- W780562451 hasConceptScore W780562451C2776748224 @default.
- W780562451 hasConceptScore W780562451C2777563447 @default.
- W780562451 hasConceptScore W780562451C2780237616 @default.
- W780562451 hasConceptScore W780562451C49105822 @default.
- W780562451 hasConceptScore W780562451C54355233 @default.
- W780562451 hasConceptScore W780562451C86803240 @default.
- W780562451 hasConceptScore W780562451C89423630 @default.
- W780562451 hasIssue "4" @default.
- W780562451 hasLocation W7805624511 @default.
- W780562451 hasOpenAccess W780562451 @default.
- W780562451 hasPrimaryLocation W7805624511 @default.
- W780562451 hasRelatedWork W1792061597 @default.
- W780562451 hasRelatedWork W2036391059 @default.
- W780562451 hasRelatedWork W2043221018 @default.
- W780562451 hasRelatedWork W2117564247 @default.
- W780562451 hasRelatedWork W2225325914 @default.
- W780562451 hasRelatedWork W2365260205 @default.
- W780562451 hasRelatedWork W2391843807 @default.
- W780562451 hasRelatedWork W2763118743 @default.
- W780562451 hasRelatedWork W2901754728 @default.
- W780562451 hasRelatedWork W2922420572 @default.
- W780562451 hasRelatedWork W2940259631 @default.
- W780562451 hasRelatedWork W2987981972 @default.
- W780562451 hasRelatedWork W2992586185 @default.
- W780562451 hasRelatedWork W2996582508 @default.
- W780562451 hasRelatedWork W2999129524 @default.
- W780562451 hasRelatedWork W3043180687 @default.
- W780562451 hasRelatedWork W213149785 @default.
- W780562451 hasRelatedWork W2845505352 @default.
- W780562451 hasRelatedWork W2866442912 @default.
- W780562451 hasRelatedWork W2988785239 @default.
- W780562451 hasVolume "45" @default.
- W780562451 isParatext "false" @default.
- W780562451 isRetracted "false" @default.
- W780562451 magId "780562451" @default.
- W780562451 workType "article" @default.