Matches in SemOpenAlex for { <https://semopenalex.org/work/W78340078> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W78340078 abstract "Aim Luminex Single Antigen (LSA) technology for antibody identification has enabled laboratories to provide highly accurate virtual crossmatch results to surgeons prior to renal transplantation. In our laboratory, unacceptable antigens (UAs) are assigned for antibodies with 1500 MFI or higher. A deceased donor offer was extended to a patient on our waitlist, and the virtual crossmatch was negative. Surprisingly, the actual flow crossmatch was strongly positive for both T cells and B cells. The organ was declined for this patient, and the discrepancy was investigated. Methods The patient was a 53 y.o. female with a prior transplant, multiple pregnancies and transfusions, and 100% cPRA. The strongest antibody this patient had to the donor (A1) had an MFI of only 1167 by LSA beads (One Lambda Inc), which was not considered a UA. In our experience, this should not have caused a positive flow crossmatch. The crossmatch was run with and without Dithiothreitol (DTT) treatment (0.05 M at 37 °C for 30 min), and the results were a Δ MESF of +123,108 (− DTT) and +86,366 (+ DTT) for T cells and +179,841 (− DTT) and +75,565 (+ DTT) for B cells. The sample had never been tested with DTT treatment on LSA beads, so the sample was run with and without DTT on LSA beads in an attempt to explain the positive crossmatch. Results The A1 bead, which had an MFI of only 1167 on LSA beads without DTT, jumped to an MFI of 17,300 with DTT for the same sample. This high MFI correlated with the strongly positive flow crossmatch, and A1 was entered as a UA for this patient. Conclusions Our laboratory had been using DTT routinely for flow crossmatches but not for antibody testing. This patient had a strong antibody to A1 that went undetected by LSA without the use of DTT. Since this discovery, our lab has begun using DTT for all LSA testing in order to more accurately predict crossmatch results." @default.
- W78340078 created "2016-06-24" @default.
- W78340078 creator A5023734584 @default.
- W78340078 creator A5088776596 @default.
- W78340078 date "2014-10-01" @default.
- W78340078 modified "2023-09-26" @default.
- W78340078 title "P021" @default.
- W78340078 doi "https://doi.org/10.1016/j.humimm.2014.08.083" @default.
- W78340078 hasPublicationYear "2014" @default.
- W78340078 type Work @default.
- W78340078 sameAs 78340078 @default.
- W78340078 citedByCount "0" @default.
- W78340078 crossrefType "journal-article" @default.
- W78340078 hasAuthorship W78340078A5023734584 @default.
- W78340078 hasAuthorship W78340078A5088776596 @default.
- W78340078 hasConcept C126322002 @default.
- W78340078 hasConcept C147483822 @default.
- W78340078 hasConcept C159654299 @default.
- W78340078 hasConcept C181199279 @default.
- W78340078 hasConcept C185592680 @default.
- W78340078 hasConcept C188280979 @default.
- W78340078 hasConcept C203014093 @default.
- W78340078 hasConcept C2777824588 @default.
- W78340078 hasConcept C2911091166 @default.
- W78340078 hasConcept C3019514911 @default.
- W78340078 hasConcept C55493867 @default.
- W78340078 hasConcept C71924100 @default.
- W78340078 hasConceptScore W78340078C126322002 @default.
- W78340078 hasConceptScore W78340078C147483822 @default.
- W78340078 hasConceptScore W78340078C159654299 @default.
- W78340078 hasConceptScore W78340078C181199279 @default.
- W78340078 hasConceptScore W78340078C185592680 @default.
- W78340078 hasConceptScore W78340078C188280979 @default.
- W78340078 hasConceptScore W78340078C203014093 @default.
- W78340078 hasConceptScore W78340078C2777824588 @default.
- W78340078 hasConceptScore W78340078C2911091166 @default.
- W78340078 hasConceptScore W78340078C3019514911 @default.
- W78340078 hasConceptScore W78340078C55493867 @default.
- W78340078 hasConceptScore W78340078C71924100 @default.
- W78340078 hasLocation W783400781 @default.
- W78340078 hasOpenAccess W78340078 @default.
- W78340078 hasPrimaryLocation W783400781 @default.
- W78340078 hasRelatedWork W137802566 @default.
- W78340078 hasRelatedWork W188280101 @default.
- W78340078 hasRelatedWork W188672157 @default.
- W78340078 hasRelatedWork W1969231794 @default.
- W78340078 hasRelatedWork W2093064127 @default.
- W78340078 hasRelatedWork W239363782 @default.
- W78340078 hasRelatedWork W2513180073 @default.
- W78340078 hasRelatedWork W2514536589 @default.
- W78340078 hasRelatedWork W2517488432 @default.
- W78340078 hasRelatedWork W2518316072 @default.
- W78340078 hasRelatedWork W254756369 @default.
- W78340078 hasRelatedWork W2560559268 @default.
- W78340078 hasRelatedWork W2751890267 @default.
- W78340078 hasRelatedWork W2753171373 @default.
- W78340078 hasRelatedWork W2790327580 @default.
- W78340078 hasRelatedWork W2889231330 @default.
- W78340078 hasRelatedWork W2889282774 @default.
- W78340078 hasRelatedWork W2908072364 @default.
- W78340078 hasRelatedWork W95050279 @default.
- W78340078 hasRelatedWork W9819961 @default.
- W78340078 isParatext "false" @default.
- W78340078 isRetracted "false" @default.
- W78340078 magId "78340078" @default.
- W78340078 workType "article" @default.