FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W785632874> ?p ?o ?g. }

• W785632874 endingPage "3380" @default.
• W785632874 startingPage "3370" @default.
• W785632874 abstract "LH/human chorionic gonadotropin receptor (LHR) undergoes down-regulation during preovulatory LH surge or in response to exposure to a supraphysiological concentration of its ligands through a posttranscriptional mechanism involving an RNA binding protein designated as LHR mRNA binding protein (LRBP). miR-122, a short noncoding RNA, has been shown to mediate the up-regulation of LRBP. In the present study, we show that inhibition of miR-122 using a locked nucleic acid (LNA)-conjugated antagomir suppressed human chorionic gonadotropin (hCG)-induced up-regulation of LRBP as well as its association with LHR mRNA, as analyzed by RNA EMSA. Most importantly, inhibition of miR-122 resulted in the abolishment of hCG-mediated LHR mRNA down-regulation. We also show that the transcription factor, sterol regulatory element binding protein (SREBP) (SREBP-1a and SREBP-2 isoforms), is an intermediate in miR-122-mediated LHR mRNA regulation. HCG-stimulated increase in the activation of both SREBP-1a and SREBP-2 was inhibited by pretreatment with the miR-122 antagomir. The inhibition of cAMP/protein kinase A (PKA) and ERK pathways, upstream activators of miR-122, abolished SREBP activation after hCG treatment. SREBP-mediated regulation of LRBP expression is mediated by recruitment of LRBP promoter element to SREBP-1a, because chromatin immunoprecipitation assay revealed that association of LRBP promoter to SREBP was increased by hCG treatment. Pretreatment with miR-122 antagomir suppressed this response. Inhibition of SREBP activation by pretreating the rats with a chemical compound, fatostatin abrogated hCG-induced up-regulation of LRBP mRNA and protein. Fatostatin also inhibited LHR-LRBP mRNA-protein complex formation and LHR down-regulation. These results conclusively show that miR-122 plays a regulatory role in LH/hCG-induced LHR mRNA down-regulation by increasing LRBP expression through the activation of SREBP pathway." @default.
• W785632874 created "2016-06-24" @default.
• W785632874 creator A5001627275 @default.
• W785632874 creator A5003194620 @default.
• W785632874 creator A5038672569 @default.
• W785632874 date "2015-09-01" @default.
• W785632874 modified "2023-10-09" @default.
• W785632874 title "miR-122 Regulates LH Receptor Expression by Activating Sterol Response Element Binding Protein in Rat Ovaries" @default.
• W785632874 cites W1875102167 @default.
• W785632874 cites W1942407231 @default.
• W785632874 cites W1965759870 @default.
• W785632874 cites W1966451804 @default.
• W785632874 cites W1971455958 @default.
• W785632874 cites W1977577386 @default.
• W785632874 cites W1990465580 @default.
• W785632874 cites W1994850049 @default.
• W785632874 cites W1996837389 @default.
• W785632874 cites W2003103474 @default.
• W785632874 cites W2012527285 @default.
• W785632874 cites W2015978359 @default.
• W785632874 cites W2017382801 @default.
• W785632874 cites W2021191487 @default.
• W785632874 cites W2022687771 @default.
• W785632874 cites W2026697437 @default.
• W785632874 cites W2029426792 @default.
• W785632874 cites W2047499053 @default.
• W785632874 cites W2047730604 @default.
• W785632874 cites W2051830269 @default.
• W785632874 cites W2052636645 @default.
• W785632874 cites W2054477606 @default.
• W785632874 cites W2062174925 @default.
• W785632874 cites W2064697073 @default.
• W785632874 cites W2064706344 @default.
• W785632874 cites W2077809517 @default.
• W785632874 cites W2080089036 @default.
• W785632874 cites W2090638971 @default.
• W785632874 cites W2091443652 @default.
• W785632874 cites W2093909923 @default.
• W785632874 cites W2097632838 @default.
• W785632874 cites W2101606263 @default.
• W785632874 cites W2106801327 @default.
• W785632874 cites W2114202720 @default.
• W785632874 cites W2116114174 @default.
• W785632874 cites W2117391818 @default.
• W785632874 cites W2130612526 @default.
• W785632874 cites W2139342290 @default.
• W785632874 cites W2141379557 @default.
• W785632874 cites W2146544786 @default.
• W785632874 cites W2161693225 @default.
• W785632874 cites W2164286307 @default.
• W785632874 cites W2171820139 @default.
• W785632874 doi "https://doi.org/10.1210/en.2015-1121" @default.
• W785632874 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4541618" @default.
• W785632874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26125464" @default.
• W785632874 hasPublicationYear "2015" @default.
• W785632874 type Work @default.
• W785632874 sameAs 785632874 @default.
• W785632874 citedByCount "18" @default.
• W785632874 countsByYear W7856328742017 @default.
• W785632874 countsByYear W7856328742018 @default.
• W785632874 countsByYear W7856328742019 @default.
• W785632874 countsByYear W7856328742021 @default.
• W785632874 countsByYear W7856328742023 @default.
• W785632874 crossrefType "journal-article" @default.
• W785632874 hasAuthorship W785632874A5001627275 @default.
• W785632874 hasAuthorship W785632874A5003194620 @default.
• W785632874 hasAuthorship W785632874A5038672569 @default.
• W785632874 hasBestOaLocation W7856328741 @default.
• W785632874 hasConcept C101762097 @default.
• W785632874 hasConcept C104317684 @default.
• W785632874 hasConcept C105580179 @default.
• W785632874 hasConcept C126322002 @default.
• W785632874 hasConcept C132376346 @default.
• W785632874 hasConcept C134018914 @default.
• W785632874 hasConcept C134320426 @default.
• W785632874 hasConcept C150194340 @default.
• W785632874 hasConcept C153911025 @default.
• W785632874 hasConcept C185592680 @default.
• W785632874 hasConcept C2775948956 @default.
• W785632874 hasConcept C2778163477 @default.
• W785632874 hasConcept C2778718757 @default.
• W785632874 hasConcept C55493867 @default.
• W785632874 hasConcept C71315377 @default.
• W785632874 hasConcept C71924100 @default.
• W785632874 hasConcept C85051948 @default.
• W785632874 hasConcept C86803240 @default.
• W785632874 hasConcept C95444343 @default.
• W785632874 hasConceptScore W785632874C101762097 @default.
• W785632874 hasConceptScore W785632874C104317684 @default.
• W785632874 hasConceptScore W785632874C105580179 @default.
• W785632874 hasConceptScore W785632874C126322002 @default.
• W785632874 hasConceptScore W785632874C132376346 @default.
• W785632874 hasConceptScore W785632874C134018914 @default.
• W785632874 hasConceptScore W785632874C134320426 @default.
• W785632874 hasConceptScore W785632874C150194340 @default.
• W785632874 hasConceptScore W785632874C153911025 @default.
• W785632874 hasConceptScore W785632874C185592680 @default.
• W785632874 hasConceptScore W785632874C2775948956 @default.

• next