Matches in SemOpenAlex for { <https://semopenalex.org/work/W786123725> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W786123725 abstract "The B mating type locus of the basidiomycete Coprinus cinereus contains a multiallelic gene complex encoding peptide pheromones and transmembrane receptors. There are an estimated 79 B mating specificities in C. cinereus , any two of which are sufficient to promote B-regulated development following cell fusion. The isolation of the B42 locus is described along with the DNA sequence analysis that identified nine B mating type genes within a 27kb B42 -specific DNA sequence. Six of the genes, with small transcripts of 800-900nt, encode the mating pheromone precursors and the other three, with 1.9 to 2-5kb transcripts, encode the transmembrane pheromone receptors. The genes are arranged in three groups, designated group 1, 2 and 3, each consisting of one receptor gene and two pheromone genes. B42 and B6 share the same alleles of the group 1 genes, but not those of groups 2 and 3. This was demonstrated by DNAsequence analysis and Southern blot analysis. None of the group 1 genes from B42 were able to activate B -regulated development in a B6 host when introduced by transformation but with one exception, all genes from group 2 and group 3 were able to do so. This analysis led to the recognition that the three genes in any one group are held together in an allele-specific DNA sequence and that Southern blot analysis and transformation can be used to identify shared alleles in uncloned loci. Extensive Southern analyses using cloned genes to probe genomic DNAs from strains having other B mating specificites showed that different B loci may share identical alleles of two groups of genes. Mating partners thus require different alleles of only one group of genes to generate a compatible B mating interaction. Transformation analyses with the same cloned genes confirmed the conclusions derived from the hybridisation data. Multiple B mating specificities thus appear to be derived from three groups of multiallelic and functionally redundant genes. A tenth gene located within the B42- specific DNA sequence encodes a putative transporter protein belonging to the major facilitator superfamily (MFS). In other genomic backgrounds this gene lies in homologous flanking sequences and its presence within the B42 locus is unlikely to be related to mating type function." @default.
- W786123725 created "2016-06-24" @default.
- W786123725 creator A5011538452 @default.
- W786123725 date "1997-01-01" @default.
- W786123725 modified "2023-09-28" @default.
- W786123725 title "Isolation and characterisation of the B42 mating type locus of Coprinus cinereus" @default.
- W786123725 hasPublicationYear "1997" @default.
- W786123725 type Work @default.
- W786123725 sameAs 786123725 @default.
- W786123725 citedByCount "0" @default.
- W786123725 crossrefType "dissertation" @default.
- W786123725 hasAuthorship W786123725A5011538452 @default.
- W786123725 hasConcept C104317684 @default.
- W786123725 hasConcept C133307689 @default.
- W786123725 hasConcept C198348125 @default.
- W786123725 hasConcept C54355233 @default.
- W786123725 hasConcept C61053724 @default.
- W786123725 hasConcept C84597430 @default.
- W786123725 hasConcept C86803240 @default.
- W786123725 hasConceptScore W786123725C104317684 @default.
- W786123725 hasConceptScore W786123725C133307689 @default.
- W786123725 hasConceptScore W786123725C198348125 @default.
- W786123725 hasConceptScore W786123725C54355233 @default.
- W786123725 hasConceptScore W786123725C61053724 @default.
- W786123725 hasConceptScore W786123725C84597430 @default.
- W786123725 hasConceptScore W786123725C86803240 @default.
- W786123725 hasLocation W7861237251 @default.
- W786123725 hasOpenAccess W786123725 @default.
- W786123725 hasPrimaryLocation W7861237251 @default.
- W786123725 hasRelatedWork W1481779862 @default.
- W786123725 hasRelatedWork W1820552278 @default.
- W786123725 hasRelatedWork W1945784894 @default.
- W786123725 hasRelatedWork W1998915296 @default.
- W786123725 hasRelatedWork W1999553924 @default.
- W786123725 hasRelatedWork W2000475723 @default.
- W786123725 hasRelatedWork W2015778105 @default.
- W786123725 hasRelatedWork W2030608039 @default.
- W786123725 hasRelatedWork W2066049288 @default.
- W786123725 hasRelatedWork W2089511860 @default.
- W786123725 hasRelatedWork W2093041540 @default.
- W786123725 hasRelatedWork W2095436721 @default.
- W786123725 hasRelatedWork W2135509485 @default.
- W786123725 hasRelatedWork W2136059685 @default.
- W786123725 hasRelatedWork W214746792 @default.
- W786123725 hasRelatedWork W2149565814 @default.
- W786123725 hasRelatedWork W2161326099 @default.
- W786123725 hasRelatedWork W2164679491 @default.
- W786123725 hasRelatedWork W239733503 @default.
- W786123725 hasRelatedWork W3206938835 @default.
- W786123725 isParatext "false" @default.
- W786123725 isRetracted "false" @default.
- W786123725 magId "786123725" @default.
- W786123725 workType "dissertation" @default.