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- W786211164 abstract "Asparagus officinalis (cultivar Franklim) was successfully propagated in vitro on a modified Murashige and Skoog medium (MMS) to which 0.1 mg dm− 3 NAA and 0.1 or 0.2 mg dm− 3 kinetin had been added. Vigorous shoot cultures were initiated by using buds and shoot apex explants. The cultures were incubated at 27 °C and a 16/8-h light/dark period. The highest percentage rooting was obtained by using a MMS medium supplemented with 0.1 mg dm− 3 NAA, 0.1 mg dm− 3 kinetin and 1.25 mg dm− 3 ancymidol. Prior to transplanting to soil, the plants were first hardened off by transferring them to basic Murashige and Skoog medium (MS) with a lower sucrose concentration, or lacking the latter, and simultaneously exposing them to a higher light intensity of 62.5 μE m− 2 s− 1. Asparagus officinalis (kultivar Franklim) is suksesvol op ’n gemodifiseerde Murashige en Skoog-medium (MMS), waarby 0.1 mg dm− 3 NAA en 0.1 of 0.2 mg dm− 3 kinetien in vitro toegevoeg is, vermeerder. Sterk loot-kulture is met die ogies en groeipunte as eksplantmateriaal verkry. Die kulture is by 27 °C en ’n 16/8-h lig/donker periode geïnkubeer. Die plantjies is op MMS-medium, aangevul met 0.1 mg dm− 3 NAA, 0.1 mg dm− 3 kinetien en 1.25 mg dm− 3 ansimidol, bewortel. Die plante is eers afgehard voordat dit in grond geplant is, deur die plante na MS-medium met ’n laer sukrosekonsentrasie oor te plaas, of deur lg. weg te laat en dit terselfdertyd aan ’n hoër ligintensiteit van 62.5 μE m− 2 s− 1 bloot te stel." @default.
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- W786211164 date "1990-06-01" @default.
- W786211164 modified "2023-09-24" @default.
- W786211164 title "Rapid in vitro multiplication of Asparagus" @default.
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- W786211164 doi "https://doi.org/10.1016/s0254-6299(16)31061-4" @default.
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