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- W78644646 abstract "Storage of cottonseed with LN for long periods of time would prevent having to replace valuable germplasm stocks except when they are required for breeding or research purposes. Seeds of eight cultivars of Gossypium hirsutum L. were stored either above or immersed under liquid nitrogen (LN) and then returned to room temperature and evaluated for quality on the basis of germination, vigor, and field development. Storage above LN at -150°C for 180 days did not appreciably affect germination and vigor and seedlings from the LN-treated seeds appeared normal. Treated and control cottonseed grown under field conditions had similar stands. The best seed moisture levels for LN storage, acccording to germination and vigor tests, were between 4 and 12%. A significant decrease in germination and vigor was observed at moisture levels above 12%. Immersion of cottonseed under LN often caused severe damage to cotyledons that was apparent during seedling development. These cottonseed germinated well on germination towels after removal from under LN, but their seedlings often did not grow as well as controls under growth chamber conditions. The present cryogenic studies indicate that storage above but not directly in LN may be suitable for long-term preservation of cotton germplasm." @default.
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- W78644646 date "2000-01-01" @default.
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- W78644646 title "Cryostorage of cottonseed with liquid nitrogen" @default.
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