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- W788402654 abstract "在低成本的聚甲基丙烯酸甲酯(PMMA)电泳芯片上,利用双通道共聚焦激光诱导荧光检测系统实现了单链DNA快速高效的分离检测.选用0.8mm厚度的PMMA薄片加工微管道,一方面降低了检测限,另一方面提高了散热性能.通过线性聚丙烯酰胺筛分胶液以及使用纤维素衍生物对微管道表面进行动态修饰等条件的优化,芯片完成了高分辨率、高重现性的短串联重复序列(STR)等位基因的快速分型检测.两个STR位点D13S317和CSFIPO的等位基因分型标准物(allelic ladder)和实际样本的PCR扩增产物均在3min内达到了基线分离,表明低成本的PMMA电泳芯片在法医学及临床医学领域的基因分析方面具有良好的发展潜能." @default.
- W788402654 created "2016-06-24" @default.
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- W788402654 date "2009-01-01" @default.
- W788402654 modified "2023-09-28" @default.
- W788402654 title "基于低成本聚甲基丙烯酸甲酯(PMMA)电泳芯片的微型DNA分析仪" @default.
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