FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W78862461> ?p ?o ?g. }

• W78862461 endingPage "130" @default.
• W78862461 startingPage "109" @default.
• W78862461 abstract "Transgenic cotton has widely been employed both in commercial cultivation and basic research. It is essential to determine which plants contain the transgene and in how many copies after transgenic cotton plants are generated. A TaqMan quantitative real-time polymerase chain reaction (Tq RT-PCR) method is described here to examine transgene copy number in transgenic cotton plants. The estimation of two transgene elements, the target gene of green fluorescence protein (GFP) and the selective gene of neomycin phosphotransferase II (NPTII), is used as an example to detail each step in Tq RT-PCR procedure, including endogenous reference gene selection, reference plasmid construction, primer-probe design, DNA extraction, real-time PCR, and data analysis. Comparing with traditional approach-Southern hybridization ­analysis, this method can be used efficiently in screening large number of T0 transgenic cotton plants at early stage of transformation process as well as identifying transgene homozygotes in a segregation population." @default.
• W78862461 created "2016-06-24" @default.
• W78862461 creator A5056243974 @default.
• W78862461 creator A5058605138 @default.
• W78862461 date "2012-10-16" @default.
• W78862461 modified "2023-10-05" @default.
• W78862461 title "Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR" @default.
• W78862461 cites W1514046652 @default.
• W78862461 cites W1661448904 @default.
• W78862461 cites W1721868265 @default.
• W78862461 cites W1966637572 @default.
• W78862461 cites W1968785061 @default.
• W78862461 cites W1969553476 @default.
• W78862461 cites W1993459319 @default.
• W78862461 cites W1996304552 @default.
• W78862461 cites W1998234438 @default.
• W78862461 cites W2009728322 @default.
• W78862461 cites W2010651176 @default.
• W78862461 cites W2011126620 @default.
• W78862461 cites W2018197799 @default.
• W78862461 cites W2024315578 @default.
• W78862461 cites W2026710381 @default.
• W78862461 cites W2032526276 @default.
• W78862461 cites W2044032739 @default.
• W78862461 cites W2051669308 @default.
• W78862461 cites W2059403782 @default.
• W78862461 cites W2091938644 @default.
• W78862461 cites W2092147415 @default.
• W78862461 cites W2100808809 @default.
• W78862461 cites W2125795583 @default.
• W78862461 cites W2146224715 @default.
• W78862461 cites W2156021383 @default.
• W78862461 cites W2165133812 @default.
• W78862461 cites W2170081920 @default.
• W78862461 doi "https://doi.org/10.1007/978-1-62703-212-4_9" @default.
• W78862461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30543067" @default.
• W78862461 hasPublicationYear "2012" @default.
• W78862461 type Work @default.
• W78862461 sameAs 78862461 @default.
• W78862461 citedByCount "5" @default.
• W78862461 countsByYear W788624612015 @default.
• W78862461 countsByYear W788624612019 @default.
• W78862461 countsByYear W788624612020 @default.
• W78862461 countsByYear W788624612021 @default.
• W78862461 countsByYear W788624612022 @default.
• W78862461 crossrefType "book-chapter" @default.
• W78862461 hasAuthorship W78862461A5056243974 @default.
• W78862461 hasAuthorship W78862461A5058605138 @default.
• W78862461 hasConcept C102230213 @default.
• W78862461 hasConcept C104317684 @default.
• W78862461 hasConcept C142613039 @default.
• W78862461 hasConcept C143660080 @default.
• W78862461 hasConcept C144024400 @default.
• W78862461 hasConcept C149923435 @default.
• W78862461 hasConcept C153911025 @default.
• W78862461 hasConcept C178790620 @default.
• W78862461 hasConcept C185592680 @default.
• W78862461 hasConcept C204241405 @default.
• W78862461 hasConcept C22744801 @default.
• W78862461 hasConcept C2777563447 @default.
• W78862461 hasConcept C2908647359 @default.
• W78862461 hasConcept C48023723 @default.
• W78862461 hasConcept C49105822 @default.
• W78862461 hasConcept C54355233 @default.
• W78862461 hasConcept C86803240 @default.
• W78862461 hasConceptScore W78862461C102230213 @default.
• W78862461 hasConceptScore W78862461C104317684 @default.
• W78862461 hasConceptScore W78862461C142613039 @default.
• W78862461 hasConceptScore W78862461C143660080 @default.
• W78862461 hasConceptScore W78862461C144024400 @default.
• W78862461 hasConceptScore W78862461C149923435 @default.
• W78862461 hasConceptScore W78862461C153911025 @default.
• W78862461 hasConceptScore W78862461C178790620 @default.
• W78862461 hasConceptScore W78862461C185592680 @default.
• W78862461 hasConceptScore W78862461C204241405 @default.
• W78862461 hasConceptScore W78862461C22744801 @default.
• W78862461 hasConceptScore W78862461C2777563447 @default.
• W78862461 hasConceptScore W78862461C2908647359 @default.
• W78862461 hasConceptScore W78862461C48023723 @default.
• W78862461 hasConceptScore W78862461C49105822 @default.
• W78862461 hasConceptScore W78862461C54355233 @default.
• W78862461 hasConceptScore W78862461C86803240 @default.
• W78862461 hasLocation W788624611 @default.
• W78862461 hasLocation W788624612 @default.
• W78862461 hasOpenAccess W78862461 @default.
• W78862461 hasPrimaryLocation W788624611 @default.
• W78862461 hasRelatedWork W1571771606 @default.
• W78862461 hasRelatedWork W1979498904 @default.
• W78862461 hasRelatedWork W1985154731 @default.
• W78862461 hasRelatedWork W1995208043 @default.
• W78862461 hasRelatedWork W2010668679 @default.
• W78862461 hasRelatedWork W2086745641 @default.
• W78862461 hasRelatedWork W2143536919 @default.
• W78862461 hasRelatedWork W2384870900 @default.
• W78862461 hasRelatedWork W2524850911 @default.
• W78862461 hasRelatedWork W4233844374 @default.
• W78862461 isParatext "false" @default.
• W78862461 isRetracted "false" @default.

• next