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- W78879418 abstract "It has been shown that: (a) the EPR-detectable distribution of a spin-labeled weak acid provides an accurate method to determine the intravesicular pH in H. halobium, whereas a spin-labeled amine is of more limited utility for such measurements because spectra obtained may need to be corrected by computer subtraction methods for amounts of the probe that are membrane bound; (b) precise quantitation of the pH gradient necessitates the simultaneous determination of the intravesicular volume, which is readily measured as the ferricyanide-inaccessible space using the spin probe Tempone; (c) spin probe movement rapidly monitors light-induced cation transport, provided this movement is linked to or causes proton movement. The quantitative data obtained with this technique reveal a maximum light-induced pH gradient of two units across H. halobium membranes at acidic bulk pH values." @default.
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- W78879418 date "1982-01-01" @default.
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- W78879418 title "[43] Light-induced pH gradients measured with spin-labeled amine and carboxylic acid probes: Application to Halobacterium halobium cell envelope vesicles" @default.
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- W78879418 doi "https://doi.org/10.1016/0076-6879(82)88046-4" @default.
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