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W789768852 abstract "AIM： To develop a cell culture system capable of producing high titer hepatitis C virus (HCV) stocks with recombinant vaccinia viruses as helpers.METHODS: Two plasmids were used for the generation of recombinant HCV: one containing the full-length HCV cDNA cloned between T7 promoter and T7 terminator of pOCUS-T7 vector, and the other containing the HCV polyprotein open reading frame (ORF) directly linked to a vaccinia late promoter in PSC59. These two plasmids were co-transfected into BHK21 cells, which were then infected with vTF7-3 recombinant vaccinia helper viruses.RESULTS: After 5 d of incubation, approximately 3.6×10^7 copies of HCV RNA were present per milliliter of cell culture supematant, as detected by fluorescence quantitative RT-PCR(FQ-PCR). The yield of recombinant HCV using this cell system increased 100- to 1000- fold compared to in vitrotranscribed HCV genomic RNA or selective subgenomic HCV RNA molecule method.CONCLUSION: This cell culture system is capable of producing high titer recombinant HCV." @default.
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W789768852 date "2004-01-01" @default.
W789768852 modified "2023-09-23" @default.
W789768852 title "A vaccinia replication system for producing recombinant hepatitis C virus" @default.
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