Matches in SemOpenAlex for { <https://semopenalex.org/work/W78977215> ?p ?o ?g. }
- W78977215 endingPage "4195" @default.
- W78977215 startingPage "4187" @default.
- W78977215 abstract "Abstract Earlier, we described a stromal cell-free two-step clonal culture system in which murine primitive lymphohematopoietic progenitors produce myeloid and B-lymphoid lineage cells. In the same culture T-cell potential of the progenitors was maintained. We now report that, in addition to myeloid and B-lymphoid cells, putative T-cell progenitors are also produced in culture. Lineage-negative (Lin−) Ly-6A/E+ c-kit+ bone marrow cells from 5-fluorouracil–treated mice were cultured in methylcellulose in the presence of SF (Steel factor), interleukin (IL)-11, and IL-7, and the resulting primary colonies were picked and pooled. When injected into severe combined immune deficiency (scid) mice, the pooled cells reconstituted the T-cell compartment of the scid mice earlier than freshly prepared primitive marrow cells. This reconstitution activity of the pooled primary colony cells was enriched in the Ly-6A/E+ and FcγRII/III−/low cell fractions. Reverse transcriptase-polymerase chain reaction (RT-PCR) and DNA-PCR analyses showed that some of the primary colony cells are differentiated sufficiently to express messenger RNA (mRNA) of T-cell receptor (TCR) β-chain and pre-TCR alpha (pT) and, although not frequently, to perform Dβ-Jβ rearrangement of the TCR gene. Micromanipulation studies confirmed the clonal origin of myeloid lineage cells and the cells positive for the T-cell–specific transcripts and D-J rearrangement of TCR β-chain. These results suggested that, in the presence of SF, IL-11, and IL-7, primitive lymphohematopoietic progenitors differentiate toward T-cell lineage in addition to myeloid and B-cell lineages." @default.
- W78977215 created "2016-06-24" @default.
- W78977215 creator A5031036213 @default.
- W78977215 creator A5040779737 @default.
- W78977215 creator A5044347721 @default.
- W78977215 creator A5048446590 @default.
- W78977215 creator A5064561599 @default.
- W78977215 date "1999-06-15" @default.
- W78977215 modified "2023-09-28" @default.
- W78977215 title "Differentiation in Culture of Murine Primitive Lymphohematopoietic Progenitors Toward T-Cell Lineage" @default.
- W78977215 cites W1488535442 @default.
- W78977215 cites W1507229894 @default.
- W78977215 cites W1514820327 @default.
- W78977215 cites W1529971420 @default.
- W78977215 cites W1534222607 @default.
- W78977215 cites W1538729663 @default.
- W78977215 cites W154745092 @default.
- W78977215 cites W1553503189 @default.
- W78977215 cites W1556496127 @default.
- W78977215 cites W1570667303 @default.
- W78977215 cites W1571577428 @default.
- W78977215 cites W1574646363 @default.
- W78977215 cites W1646719553 @default.
- W78977215 cites W1678194206 @default.
- W78977215 cites W1926376253 @default.
- W78977215 cites W1969542161 @default.
- W78977215 cites W1971833736 @default.
- W78977215 cites W1972044265 @default.
- W78977215 cites W1976861055 @default.
- W78977215 cites W197701292 @default.
- W78977215 cites W1981441452 @default.
- W78977215 cites W1988855837 @default.
- W78977215 cites W1993527476 @default.
- W78977215 cites W1993641677 @default.
- W78977215 cites W1995179449 @default.
- W78977215 cites W1996822762 @default.
- W78977215 cites W2008661167 @default.
- W78977215 cites W2017291374 @default.
- W78977215 cites W2019513091 @default.
- W78977215 cites W2022438537 @default.
- W78977215 cites W2022842992 @default.
- W78977215 cites W2023236971 @default.
- W78977215 cites W2031179453 @default.
- W78977215 cites W2034403218 @default.
- W78977215 cites W2040721100 @default.
- W78977215 cites W2065388713 @default.
- W78977215 cites W2066790739 @default.
- W78977215 cites W2067194336 @default.
- W78977215 cites W2070437307 @default.
- W78977215 cites W2071142530 @default.
- W78977215 cites W2073585051 @default.
- W78977215 cites W2080801585 @default.
- W78977215 cites W2080809922 @default.
- W78977215 cites W2082492626 @default.
- W78977215 cites W2085555998 @default.
- W78977215 cites W208730735 @default.
- W78977215 cites W2087832707 @default.
- W78977215 cites W2092680301 @default.
- W78977215 cites W2096632716 @default.
- W78977215 cites W2127324955 @default.
- W78977215 cites W2139216153 @default.
- W78977215 cites W2141231618 @default.
- W78977215 cites W2141666411 @default.
- W78977215 cites W2152414109 @default.
- W78977215 cites W2154029983 @default.
- W78977215 cites W2156423848 @default.
- W78977215 cites W2162743131 @default.
- W78977215 cites W2164488073 @default.
- W78977215 cites W2168990989 @default.
- W78977215 cites W2171240903 @default.
- W78977215 cites W2281631393 @default.
- W78977215 cites W232671765 @default.
- W78977215 cites W2395197571 @default.
- W78977215 cites W2409089508 @default.
- W78977215 cites W2430335575 @default.
- W78977215 cites W58481624 @default.
- W78977215 doi "https://doi.org/10.1182/blood.v93.12.4187" @default.
- W78977215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10361116" @default.
- W78977215 hasPublicationYear "1999" @default.
- W78977215 type Work @default.
- W78977215 sameAs 78977215 @default.
- W78977215 citedByCount "11" @default.
- W78977215 crossrefType "journal-article" @default.
- W78977215 hasAuthorship W78977215A5031036213 @default.
- W78977215 hasAuthorship W78977215A5040779737 @default.
- W78977215 hasAuthorship W78977215A5044347721 @default.
- W78977215 hasAuthorship W78977215A5048446590 @default.
- W78977215 hasAuthorship W78977215A5064561599 @default.
- W78977215 hasConcept C104317684 @default.
- W78977215 hasConcept C109159458 @default.
- W78977215 hasConcept C113646796 @default.
- W78977215 hasConcept C131665280 @default.
- W78977215 hasConcept C148738053 @default.
- W78977215 hasConcept C153911025 @default.
- W78977215 hasConcept C156695909 @default.
- W78977215 hasConcept C16930146 @default.
- W78977215 hasConcept C19317047 @default.
- W78977215 hasConcept C201750760 @default.