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- W791396223 abstract "[Objective] To develop an indirect sandwich ELISA for rapid detection of duck swollen head septicemia virus (DSHSV). [Method] DSHSV hyperimmune sera were prepared in ducks and rabbits by inoculation with DSHSV XD strain and then purified using saturated ammonium sulfate and Sephadex G-150 column chromatography to obtain anti-DSHSV IgG. An indirect sandwich ELISA was developed using the purified duck anti-DSHSV IgG and rabbit anti-DSHSV IgG after reaction conditions were optimized. Its specificity, sensitivity and repeatability were evaluated, and its accuracy was confirmed by observation with immunoelectron microscopy. Then, distribution of DSHSV in tissues of challenged ducks was also detected. [Result] Through optimizing conditions, the ELISA was developed. Only DSHSV could be detected by the developed method, but other pathogens could not be detected. Compared with agar gel diffusion test, the developed method was more sensitive. The coefficient of variation was less than 10%, and the developed method had good repeatability. In addition, the ELISA-positive samples contained DSHSV, as confirmed by immunoelectron microscopy. All heart, liver, lung and kidney collected from the DSHSV-challenged ducks were positive when they were detected by the developed ELISA. [Conclusion] The developed ELISA method is rapid, simple, specific and sensitive, and it is suitable for large-scale quarantine of DSHS. Heart, liver, lung and kidney should be selected preferentially as specimens for diagnosis of DSHS." @default.
- W791396223 created "2016-06-24" @default.
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- W791396223 date "2011-01-01" @default.
- W791396223 modified "2023-09-25" @default.
- W791396223 title "Development of an Indirect Sandwich ELISA for Detection of Duck Swollen Head Septicemia Virus" @default.
- W791396223 hasPublicationYear "2011" @default.
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