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• W793516797 abstract "Identification of Blow flies (Diptera: Calliphoridae) to species is important for estimating postmortem intervals, because insect species develop at different rates. Three Blow fly species in Georgia; Lucilia coeruleiviridis, L. cuprina, and L. sericata are similar morphologically, making identification difficult. Furthermore, the status of L. cuprina is in doubt; this species may be a complex of true L. cuprina, and L. cuprina x L. sericata hybrids. The objective of this study was to survey the Georgia Blow fly community and validate the statuses of L. coeruleiviridis, L. cuprina, and L. sericata, through morphological and genetic analyses. For a representative sample, stillborn pigs were placed at five field sites in South Georgia and flies collected from each location over a 72 hour time period. Morphological identifications, to species, were performed using dichotomous keys. We found L. coeruleiviridis at all five locations, L. cuprina at two locations, and zero L. sericata. Lucilia cuprina traits were more variable than previously reported. We sequenced the COI gene for 31 Lucilia flies; 29 COI sequences confirmed morphological identifications, while 2 COI sequences did not match the morphological identifications. There were extremely low sequence differences in both L. coeruleiviridis and L. cuprina. Thus, this study concluded that although bristles, the key feature used to identify a species, are more variable than believed, morphological and genetic analyses correspond well. To ensure a high level of accuracy, however, we suggest that both morphological and genetic techniques be used to identify Lucilia species in Georgia. Thesis Mentor:________________________ Dr. Edward B. Mondor Thesis Mentor:________________________ Dr. J. Scott Harrison Honors Director:_______________________ Dr. Steven Engel April 2014 Department of Biology University Honors Program Georgia Southern University 2 Acknowledgments I would like to thank Allen Harris for providing the pigs and Amy Hulett, Jenny Brooks, and Amy Register for providing field locations. I thank the University Honors Program Undergraduate Research Fund and the Department of Biology, Georgia Southern University for funding this project. I would like to sincerely thank Dr. Edward B. Mondor and Dr. J. Scott Harrison for allowing me to work with them. You are both exceptional mentors and have taught me so many valuable skills. To Dr. Mondor: you taught me fly anatomy, methods of identification, and shared with me your interests in the field of forensic entomology. You worked with me diligently throughout this entire process and I appreciate all the help you provided me. To Dr. Harrison: you taught me the importance of good laboratory techniques along with problem solving skills. I am grateful to you for making this study a reality and not killing me in the process. To my family: thank you for all of your love and support, especially during my mood swings while I was writing this thesis. To Ashley Johns: for your proofreading. I do not know what I would do without your extra set of eyes looking everything over. Lastly to my husband, Donald, thank you for taking amazing photos for me, collecting flies with me, and loving me throughout the entire process." @default.
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• W793516797 date "2014-01-01" @default.
• W793516797 modified "2023-09-26" @default.
• W793516797 title "A Morphological and Genetic Analysis of Forensically Important Blow Flies, from Georgia: The Genus Lucilia" @default.
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