Matches in SemOpenAlex for { <https://semopenalex.org/work/W794086962> ?p ?o ?g. }
- W794086962 abstract "During maize endosperm development, cells switch from mitotic into endoreduplication cell cycles, concomitant with the differentiation of starchy endosperm cells. The identity and role of cyclins and cyclin-dependent kinases (CDKs) that control these cell cycles are poorly understood. I identified and characterized maize cyclins of the D(CYCD2;1 and CYCD5;1) and A1-types (CYCA1;3) that are expressed in endosperm. CYCA1;3 RNA decreased sharply as the endosperm transitioned from the mitotic to the endoreduplication stage, whereas CYCD2;1 and CYCD5;1 transcripts declined gradually. Polyclonal antibodies against CYCA1;3, CYCD5;1, and CYCD2;1 and a B-type homologue, CYCB1;3, were used to characterize cyclin protein accumulation, the associated CDK activity, and spatial localization of cyclins in endosperm. Except for CYCA1;3, cyclin protein levels were nearly constant or decreased slightly throughout development. CDK activity associated with CYCA1;3 was most abundant 7 days after pollination (DAP), while that associated with CYCB1;3, CYCD2;1 and CYCD5;1 peaked at 11 DAP. CYCA1;3 appeared to be most abundant in the cytoplasm of non-endoreduplicating cells. CYCB1;3 and CYCD2;1 were found throughout the endosperm, while CYCD5;1 was mostly" @default.
- W794086962 created "2016-06-24" @default.
- W794086962 creator A5019360026 @default.
- W794086962 date "2005-01-01" @default.
- W794086962 modified "2023-09-24" @default.
- W794086962 title "Characterization of cyclin-dependent kinases and their expression in developing maize endosperm" @default.
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