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- W794328641 abstract "通过DEAE-Sepharose-FF离子交换层析法，对牛背最长肌中钙激活酶（calpain）进行了分离纯化，并确定了最佳分离条件。试验分2个阶段进行离子交换线性梯度洗脱，第1阶段线性梯度为浓度0～215mm01/L NaCl，第2阶段线性梯度为浓度215～500mmol／LNaCl，洗脱流速为2mL／min，并对每一纯化步骤蛋白浓度的变化情况作出了详细的描述和说明；在波长280nm下测定洗脱液的光吸收值，洗脱得到3个蛋白峰，μ-calpain和m-calpain分别在NaCl浓度为130～200mmol／L和230～330mmol／L时被洗脱下来，钙激活酶蛋白抑制剂（calpastatin）在NaCl浓度为25-125mmol/L时被洗脱下来。calpmn的分子量约为110kDa，通过对提取液电泳试验表明，检测结果恰好在分子量约为110kDa处有一条清晰的带，说明所得提取物为calpain。" @default.
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- W794328641 date "2009-01-01" @default.
- W794328641 modified "2023-09-28" @default.
- W794328641 title "DEAE-Sepharose-FF离子交换层析对牛背最长肌中钙激活酶的纯化研究" @default.
- W794328641 hasPublicationYear "2009" @default.
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