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- W794857393 abstract "The purpose of the present study was to produce and characterize a monoclonal antibody against human β₂-adrenergic receptor. Male BALB/c mice were immunized with glutathione S-transferase (GST) fusion protein of the C-terminal portion of the human β₂-adrenergic receptor which was expressed in E.Coli. The immunized splenocytes were fused with myeloma SP2/0-Ag14 cells. The resulting hybridomas were screened for the production of a monoclonal antibody which can recognize human β₂-adrenergic receptor, and then subcloned by limiting dilution. The resulting monoclonal antibody was named as mAbβCO2. The monoclonal antibody βCO2 was determined as IgM subtype and then purified by anti-mouse IgM-agarose affinity chromatography. The results of ELISA, Western blot, and immunocytochemistry showed that mAbβCO2 recognized human β₂-adrenergic receptor in the β₂-adrenergic receptor-GST fusion protein and human epidermoid carcinoma cell line A431 with highly specific immunoreactivity. The monoclonal antibody βCO2 may provide useful tools for the study of the β-adrenergic receptor of human and other species including rats." @default.
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- W794857393 date "1997-01-01" @default.
- W794857393 modified "2023-09-23" @default.
- W794857393 title "Production and Characterization of a Monoclonal Antibody against Human ${beta}_2$-adrenergic receptor" @default.
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