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- W795000478 abstract "Insulin activates downstream pathways in skeletal muscle including PI3K and Akt. On the other hand, ATP is released by contractile activity in skeletal muscle cells and extracellular ATP is able to act as an autocrine or paracrine factor in muscle cells. We determined the effects of extracellular ATP in Akt signaling and glucose uptake. Adult muscle fibers and rat myotubes were exposed to electric field tetanic stimulation (400 pulses of 1 ms at 45 Hz), 100 nM insulin or 100 ìM ATP. Electrical stimulation induced ATP release and Akt phosphorylation in myotubes. We measured uptake of the fluorescent hexoses 2-NBDG and 6-NBDG at room temperature using confocal microscopy. The hexose dyes were excited at 488 nm and images were collected at 505–550 nm. Fluorescent images were analyzed and glucose uptake was normalized to the basal condition. Tetanic electrical stimulation, insulin and ATP were able to induce glucose uptake in both myotubes and adult fibers. ATP, before insulin addition, increased glucose uptake induced by insulin. 10 μM each Akt VIII inhibitor and LY 294002 inhibited the increase in glucose uptake in myotubes. Results suggest that electrical stimulation potentiates insulin signaling in skeletal muscle via ATP, which may play an important role as a mediator of glucose uptake during muscle activity. FONDAP 15010006, FONDECYT 11090301, AT-24100067" @default.
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- W795000478 date "2011-04-01" @default.
- W795000478 modified "2023-10-16" @default.
- W795000478 title "ATP release induced by electrical stimulation increases glucose uptake via AKT in skeletal muscle" @default.
- W795000478 doi "https://doi.org/10.1096/fasebj.25.1_supplement.914.7" @default.
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