Matches in SemOpenAlex for { <https://semopenalex.org/work/W79604192> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W79604192 abstract "SP-A plays an important role in surfactant lipid turnover in the lung. P63 (CKAP4) located on both the plasma membrane (PM) and ER of type II pneumocytes has been identified as an SP-A receptor. Interference with specific calcium (Ca++)-dependent binding of SP-A to type II cells occurs in competition studies using anti-P63 pAb and upon treatment with siRNA to reduce P63 protein levels. A 2.5-fold enhancement of 125I-SP-A Ca++-dependent binding to type II cells plated on microporous membranes was achieved by exposure of the cells to the secretagogue cAMP (0.1mM), without effect on non-specific (Ca++-independent) SP-A cell association. Anti-P63 Ab blocked the cAMP-augmented specific binding of SP-A, evidence for the importance of P63 in this process. Inhibitors of receptor recycling were utilized to evaluate whether the forward transport of the ER-resident P63 to the PM was necessary for receptor function. Monensin, a carboxylic ionophore, increases the pH of intracellular vesicles and brefeldin A (BFA) blocks formation of transport vesicles. In the presence of monensin (2uM) or BFA (10ug/ml), cAMP-augmentation of the specific binding of SP-A was abolished. Thus, secretagogue stimulation of SP-A binding required the movement of P63 protein from the ER to the PM. Our results provide further evidence for the role of P63 as a SP-A receptor protein on lung type II cells. [HL 19737]" @default.
- W79604192 created "2016-06-24" @default.
- W79604192 creator A5022854559 @default.
- W79604192 creator A5036416315 @default.
- W79604192 creator A5054698138 @default.
- W79604192 creator A5066053446 @default.
- W79604192 creator A5074027830 @default.
- W79604192 date "2009-04-01" @default.
- W79604192 modified "2023-10-16" @default.
- W79604192 title "Forward transport of the receptor P63 is necessary for secretagogue‐enhanced surfactant protein‐A (SP‐A) binding to type II pneumocytes" @default.
- W79604192 doi "https://doi.org/10.1096/fasebj.23.1_supplement.623.1" @default.
- W79604192 hasPublicationYear "2009" @default.
- W79604192 type Work @default.
- W79604192 sameAs 79604192 @default.
- W79604192 citedByCount "0" @default.
- W79604192 crossrefType "journal-article" @default.
- W79604192 hasAuthorship W79604192A5022854559 @default.
- W79604192 hasAuthorship W79604192A5036416315 @default.
- W79604192 hasAuthorship W79604192A5054698138 @default.
- W79604192 hasAuthorship W79604192A5066053446 @default.
- W79604192 hasAuthorship W79604192A5074027830 @default.
- W79604192 hasConcept C119062480 @default.
- W79604192 hasConcept C12554922 @default.
- W79604192 hasConcept C130316041 @default.
- W79604192 hasConcept C1491633281 @default.
- W79604192 hasConcept C170493617 @default.
- W79604192 hasConcept C185592680 @default.
- W79604192 hasConcept C2780161494 @default.
- W79604192 hasConcept C2780224938 @default.
- W79604192 hasConcept C2781373781 @default.
- W79604192 hasConcept C41625074 @default.
- W79604192 hasConcept C55493867 @default.
- W79604192 hasConcept C86803240 @default.
- W79604192 hasConcept C95444343 @default.
- W79604192 hasConceptScore W79604192C119062480 @default.
- W79604192 hasConceptScore W79604192C12554922 @default.
- W79604192 hasConceptScore W79604192C130316041 @default.
- W79604192 hasConceptScore W79604192C1491633281 @default.
- W79604192 hasConceptScore W79604192C170493617 @default.
- W79604192 hasConceptScore W79604192C185592680 @default.
- W79604192 hasConceptScore W79604192C2780161494 @default.
- W79604192 hasConceptScore W79604192C2780224938 @default.
- W79604192 hasConceptScore W79604192C2781373781 @default.
- W79604192 hasConceptScore W79604192C41625074 @default.
- W79604192 hasConceptScore W79604192C55493867 @default.
- W79604192 hasConceptScore W79604192C86803240 @default.
- W79604192 hasConceptScore W79604192C95444343 @default.
- W79604192 hasIssue "S1" @default.
- W79604192 hasLocation W796041921 @default.
- W79604192 hasOpenAccess W79604192 @default.
- W79604192 hasPrimaryLocation W796041921 @default.
- W79604192 hasRelatedWork W1967792036 @default.
- W79604192 hasRelatedWork W1984650371 @default.
- W79604192 hasRelatedWork W1985982610 @default.
- W79604192 hasRelatedWork W1995895806 @default.
- W79604192 hasRelatedWork W2035477123 @default.
- W79604192 hasRelatedWork W2041420683 @default.
- W79604192 hasRelatedWork W2048809598 @default.
- W79604192 hasRelatedWork W2104625991 @default.
- W79604192 hasRelatedWork W2113776812 @default.
- W79604192 hasRelatedWork W2121439506 @default.
- W79604192 hasVolume "23" @default.
- W79604192 isParatext "false" @default.
- W79604192 isRetracted "false" @default.
- W79604192 magId "79604192" @default.
- W79604192 workType "article" @default.