Matches in SemOpenAlex for { <https://semopenalex.org/work/W796615812> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W796615812 abstract "The major cause of corneal opacity and resultant visual loss is a critical decrease in corneal endothelial cell density. Due to the fact that corneal endothelial cells do not generally proliferate, cell densities gradually decrease in the corneal endothelium with age. Before we can begin to aid patients with decreasing endothelial cell densities, by inhibiting cell death or stimulating cell proliferation, it is necessary to understand the basic cell signalling that underlies these processes. As human tissue is difficult to obtain, a representative animal model of corneal endothelium was devised. Analysis of cell morphology and expression of ct1 type VIII collagen were used to verify that primary cells derived from an explant model of mouse corneal cells were endothelial. A range of phenotypic and functional cellular features were also analysed to assess the usefulness of primary mouse corneal endothelial cultures as a model for human corneal endothelium. These included tight junction protein localization, proliferative responses to growth factor stimulation and ERK1/2 activation following growth factor stimulation. From these analyses, it was shown that primary mouse corneal endothelial cultures provide a representative model of the human corneal endothelium. A similar comparison was also made between primary mouse corneal endothelial cultures and an SV40 transformed mouse corneal endothelial cell line. Studies revealed significant differences in propensity to proliferate, junctional integrity, ERK1/2 activation, expression of apoptotic proteins and sensitivity to staurosporine-induced apoptosis between primary cells and the SV40 transformed cell line, suggesting that the SV40 transformed cell line is a less appropriate model for primary mouse corneal endothelial cells. In conclusion, the derivation and characterisation of mouse primary corneal endothelial cells provides a better model of the corneal endothelium, that offers greater understanding of cellular responses and which may eventually lead to the development of alternative therapies for primary corneal endotheliopathies." @default.
- W796615812 created "2016-06-24" @default.
- W796615812 creator A5067152490 @default.
- W796615812 date "2005-01-01" @default.
- W796615812 modified "2023-09-23" @default.
- W796615812 title "Regulation of viability in corneal endothelial cells" @default.
- W796615812 hasPublicationYear "2005" @default.
- W796615812 type Work @default.
- W796615812 sameAs 796615812 @default.
- W796615812 citedByCount "0" @default.
- W796615812 crossrefType "dissertation" @default.
- W796615812 hasAuthorship W796615812A5067152490 @default.
- W796615812 hasConcept C123012128 @default.
- W796615812 hasConcept C134018914 @default.
- W796615812 hasConcept C169760540 @default.
- W796615812 hasConcept C202751555 @default.
- W796615812 hasConcept C203014093 @default.
- W796615812 hasConcept C2775901793 @default.
- W796615812 hasConcept C2776882836 @default.
- W796615812 hasConcept C2776992346 @default.
- W796615812 hasConcept C54355233 @default.
- W796615812 hasConcept C81885089 @default.
- W796615812 hasConcept C86803240 @default.
- W796615812 hasConcept C95444343 @default.
- W796615812 hasConceptScore W796615812C123012128 @default.
- W796615812 hasConceptScore W796615812C134018914 @default.
- W796615812 hasConceptScore W796615812C169760540 @default.
- W796615812 hasConceptScore W796615812C202751555 @default.
- W796615812 hasConceptScore W796615812C203014093 @default.
- W796615812 hasConceptScore W796615812C2775901793 @default.
- W796615812 hasConceptScore W796615812C2776882836 @default.
- W796615812 hasConceptScore W796615812C2776992346 @default.
- W796615812 hasConceptScore W796615812C54355233 @default.
- W796615812 hasConceptScore W796615812C81885089 @default.
- W796615812 hasConceptScore W796615812C86803240 @default.
- W796615812 hasConceptScore W796615812C95444343 @default.
- W796615812 hasLocation W7966158121 @default.
- W796615812 hasOpenAccess W796615812 @default.
- W796615812 hasPrimaryLocation W7966158121 @default.
- W796615812 hasRelatedWork W1119896563 @default.
- W796615812 hasRelatedWork W1570369748 @default.
- W796615812 hasRelatedWork W1824598974 @default.
- W796615812 hasRelatedWork W1983057716 @default.
- W796615812 hasRelatedWork W20157305 @default.
- W796615812 hasRelatedWork W2033549785 @default.
- W796615812 hasRelatedWork W2081890326 @default.
- W796615812 hasRelatedWork W2121072212 @default.
- W796615812 hasRelatedWork W2360049993 @default.
- W796615812 hasRelatedWork W2375046233 @default.
- W796615812 hasRelatedWork W2378289224 @default.
- W796615812 hasRelatedWork W2387778536 @default.
- W796615812 hasRelatedWork W2439489431 @default.
- W796615812 hasRelatedWork W2516555097 @default.
- W796615812 hasRelatedWork W2944532049 @default.
- W796615812 hasRelatedWork W2993945395 @default.
- W796615812 hasRelatedWork W776038714 @default.
- W796615812 hasRelatedWork W903927691 @default.
- W796615812 hasRelatedWork W953268243 @default.
- W796615812 hasRelatedWork W2772708995 @default.
- W796615812 isParatext "false" @default.
- W796615812 isRetracted "false" @default.
- W796615812 magId "796615812" @default.
- W796615812 workType "dissertation" @default.