Matches in SemOpenAlex for { <https://semopenalex.org/work/W79853856> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W79853856 endingPage "144.31" @default.
- W79853856 startingPage "144.31" @default.
- W79853856 abstract "Abstract The human pathogen Streptococcus pyogenes produces various surface proteins in order to avoid the host immune defence systems. Two of these proteins, EndoS and IdeS, have proven to be very useful as tools in antibody engineering. EndoS hydrolyses the chitobiose core of the aspargine linked glycan on the heavy chain of IgG and IdeS is a cysteine protease that cleaves the IgG molecule at a unique site in the hinge region. Here we show, for the first time, that mass spectrometric analysis of antibody fragments are greatly facilitated by combining EndoS and IdeS activities. Utilization of RPC18 LC-ESI-MS generated a well-defined protein envelope with a deconvoluted mass of 24137 Da corresponding to the IgG1 heavy chain cleaved at position Gly236. This opens up new possibilities for utilizing a combination of these enzymes for fast and accurate analysis of IgG:s in a variety of mass spectrometry applications. In addition, a mutated variant of the EndoS enzyme was found to show a high affinity for IgG molecules and has been evaluated in affinity purification of intact, native IgG molecules since it excludes non-native IgG molecules. Furthermore, the affinity purification using the mutant EndoS can be performed at neutral pH which is favourable in purification of acid sensitive IgG clones. The performance and characteristics of Ides, EndoS and the EndoS mutant is highlighted during the presentation with data from several individual studies." @default.
- W79853856 created "2016-06-24" @default.
- W79853856 creator A5004735864 @default.
- W79853856 creator A5031564860 @default.
- W79853856 creator A5034599309 @default.
- W79853856 creator A5047246047 @default.
- W79853856 creator A5087338698 @default.
- W79853856 date "2010-04-01" @default.
- W79853856 modified "2023-09-24" @default.
- W79853856 title "Unique enzymes improve mass spectroscopy of IgG and make it possible to use affinity purification of acid sensitive IgG molecules (144.31)" @default.
- W79853856 doi "https://doi.org/10.4049/jimmunol.184.supp.144.31" @default.
- W79853856 hasPublicationYear "2010" @default.
- W79853856 type Work @default.
- W79853856 sameAs 79853856 @default.
- W79853856 citedByCount "0" @default.
- W79853856 crossrefType "journal-article" @default.
- W79853856 hasAuthorship W79853856A5004735864 @default.
- W79853856 hasAuthorship W79853856A5031564860 @default.
- W79853856 hasAuthorship W79853856A5034599309 @default.
- W79853856 hasAuthorship W79853856A5047246047 @default.
- W79853856 hasAuthorship W79853856A5087338698 @default.
- W79853856 hasConcept C108625454 @default.
- W79853856 hasConcept C125823703 @default.
- W79853856 hasConcept C159654299 @default.
- W79853856 hasConcept C170493617 @default.
- W79853856 hasConcept C181199279 @default.
- W79853856 hasConcept C185592680 @default.
- W79853856 hasConcept C203014093 @default.
- W79853856 hasConcept C206212055 @default.
- W79853856 hasConcept C2776714187 @default.
- W79853856 hasConcept C2779201268 @default.
- W79853856 hasConcept C2779306812 @default.
- W79853856 hasConcept C2779489039 @default.
- W79853856 hasConcept C2780898057 @default.
- W79853856 hasConcept C2909900342 @default.
- W79853856 hasConcept C523546767 @default.
- W79853856 hasConcept C54355233 @default.
- W79853856 hasConcept C55493867 @default.
- W79853856 hasConcept C86803240 @default.
- W79853856 hasConceptScore W79853856C108625454 @default.
- W79853856 hasConceptScore W79853856C125823703 @default.
- W79853856 hasConceptScore W79853856C159654299 @default.
- W79853856 hasConceptScore W79853856C170493617 @default.
- W79853856 hasConceptScore W79853856C181199279 @default.
- W79853856 hasConceptScore W79853856C185592680 @default.
- W79853856 hasConceptScore W79853856C203014093 @default.
- W79853856 hasConceptScore W79853856C206212055 @default.
- W79853856 hasConceptScore W79853856C2776714187 @default.
- W79853856 hasConceptScore W79853856C2779201268 @default.
- W79853856 hasConceptScore W79853856C2779306812 @default.
- W79853856 hasConceptScore W79853856C2779489039 @default.
- W79853856 hasConceptScore W79853856C2780898057 @default.
- W79853856 hasConceptScore W79853856C2909900342 @default.
- W79853856 hasConceptScore W79853856C523546767 @default.
- W79853856 hasConceptScore W79853856C54355233 @default.
- W79853856 hasConceptScore W79853856C55493867 @default.
- W79853856 hasConceptScore W79853856C86803240 @default.
- W79853856 hasIssue "1_Supplement" @default.
- W79853856 hasLocation W798538561 @default.
- W79853856 hasOpenAccess W79853856 @default.
- W79853856 hasPrimaryLocation W798538561 @default.
- W79853856 hasRelatedWork W1908224577 @default.
- W79853856 hasRelatedWork W1975082171 @default.
- W79853856 hasRelatedWork W1981362744 @default.
- W79853856 hasRelatedWork W2061641422 @default.
- W79853856 hasRelatedWork W2082247235 @default.
- W79853856 hasRelatedWork W2388707004 @default.
- W79853856 hasRelatedWork W3021099711 @default.
- W79853856 hasRelatedWork W3163191158 @default.
- W79853856 hasRelatedWork W4295008913 @default.
- W79853856 hasRelatedWork W79853856 @default.
- W79853856 hasVolume "184" @default.
- W79853856 isParatext "false" @default.
- W79853856 isRetracted "false" @default.
- W79853856 magId "79853856" @default.
- W79853856 workType "article" @default.