Matches in SemOpenAlex for { <https://semopenalex.org/work/W79888968> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W79888968 abstract "Plasma ceramides have been linked to peripheral insulin resistance, however the cellular mechanism is unclear. We investigated whether incubation with the long chain C14 ceramide would reduce insulin-stimulated glucose uptake in muscle cells and if so, whether it acts via insulin signaling through Akt. C2C12 muscle cells were exposed to either C6 or C14 ceramide (50 uM), or vehicle, for 1 hour. Glucose uptake was measured using 3H-2-deoxyglucose. Parallel studies using non-labeled glucose were used to determine Akt (Ser473) phosphorylation via ELISA, and cellular content of ceramide via electrospray ionization tandem mass spectrometry after separation with high-performance liquid chromatography. Insulin-stimulated glucose uptake was significantly reduced following treatment with both C6 and C14 compared to untreated cells (P=0.001). Akt phosphorylation was reduced in C6 treated cells; but was unaffected by C14 treatment. Further, there was an increase in C6 content in the cells following treatment with C6; however there was no change in C14 content following C14 treatment, suggesting that this long chain ceramide is not transported into the cell. These preliminary data suggest that C14 ceramide down-regulates insulin-stimulated glucose uptake via a different molecular mechanism than C6 ceramide, possibly a second messenger system, and is independent of insulin signaling through Akt." @default.
- W79888968 created "2016-06-24" @default.
- W79888968 creator A5008745523 @default.
- W79888968 creator A5029618902 @default.
- W79888968 creator A5040290418 @default.
- W79888968 creator A5048496897 @default.
- W79888968 creator A5061154135 @default.
- W79888968 date "2010-04-01" @default.
- W79888968 modified "2023-10-16" @default.
- W79888968 title "Exogenous long chain C14 ceramide prevents insulin‐stimulated glucose uptake in C2C12 muscle cells independent of Akt signaling" @default.
- W79888968 doi "https://doi.org/10.1096/fasebj.24.1_supplement.783.5" @default.
- W79888968 hasPublicationYear "2010" @default.
- W79888968 type Work @default.
- W79888968 sameAs 79888968 @default.
- W79888968 citedByCount "0" @default.
- W79888968 crossrefType "journal-article" @default.
- W79888968 hasAuthorship W79888968A5008745523 @default.
- W79888968 hasAuthorship W79888968A5029618902 @default.
- W79888968 hasAuthorship W79888968A5040290418 @default.
- W79888968 hasAuthorship W79888968A5048496897 @default.
- W79888968 hasAuthorship W79888968A5061154135 @default.
- W79888968 hasConcept C112446052 @default.
- W79888968 hasConcept C11960822 @default.
- W79888968 hasConcept C126322002 @default.
- W79888968 hasConcept C134018914 @default.
- W79888968 hasConcept C163235415 @default.
- W79888968 hasConcept C185592680 @default.
- W79888968 hasConcept C190283241 @default.
- W79888968 hasConcept C2777391703 @default.
- W79888968 hasConcept C2777851122 @default.
- W79888968 hasConcept C2777866211 @default.
- W79888968 hasConcept C2779306644 @default.
- W79888968 hasConcept C55493867 @default.
- W79888968 hasConcept C62478195 @default.
- W79888968 hasConcept C71924100 @default.
- W79888968 hasConcept C75217442 @default.
- W79888968 hasConcept C86803240 @default.
- W79888968 hasConceptScore W79888968C112446052 @default.
- W79888968 hasConceptScore W79888968C11960822 @default.
- W79888968 hasConceptScore W79888968C126322002 @default.
- W79888968 hasConceptScore W79888968C134018914 @default.
- W79888968 hasConceptScore W79888968C163235415 @default.
- W79888968 hasConceptScore W79888968C185592680 @default.
- W79888968 hasConceptScore W79888968C190283241 @default.
- W79888968 hasConceptScore W79888968C2777391703 @default.
- W79888968 hasConceptScore W79888968C2777851122 @default.
- W79888968 hasConceptScore W79888968C2777866211 @default.
- W79888968 hasConceptScore W79888968C2779306644 @default.
- W79888968 hasConceptScore W79888968C55493867 @default.
- W79888968 hasConceptScore W79888968C62478195 @default.
- W79888968 hasConceptScore W79888968C71924100 @default.
- W79888968 hasConceptScore W79888968C75217442 @default.
- W79888968 hasConceptScore W79888968C86803240 @default.
- W79888968 hasIssue "S1" @default.
- W79888968 hasLocation W798889681 @default.
- W79888968 hasOpenAccess W79888968 @default.
- W79888968 hasPrimaryLocation W798889681 @default.
- W79888968 hasRelatedWork W1988699731 @default.
- W79888968 hasRelatedWork W2017630799 @default.
- W79888968 hasRelatedWork W2021567090 @default.
- W79888968 hasRelatedWork W2046548573 @default.
- W79888968 hasRelatedWork W2048747321 @default.
- W79888968 hasRelatedWork W2109607186 @default.
- W79888968 hasRelatedWork W2143063964 @default.
- W79888968 hasRelatedWork W2407440476 @default.
- W79888968 hasRelatedWork W3113810870 @default.
- W79888968 hasRelatedWork W4239279690 @default.
- W79888968 hasVolume "24" @default.
- W79888968 isParatext "false" @default.
- W79888968 isRetracted "false" @default.
- W79888968 magId "79888968" @default.
- W79888968 workType "article" @default.