FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W801142006> ?p ?o ?g. }

• W801142006 abstract "Induction of germline (GL) e transcripts, an essential step preceding immunoglobulin (Ig) isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g. CD40 ligand (CD40L). AP-1 (Fos and Jun), induced transiently by CD40L, binds a DNA element in the mouse GL e promoter. AP-1 synergizes with Stat6 to activate both the intact GL e promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL e promoter. By contrast, C/EBPβ, which transactivates the human GL e promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not transactivate the human GL e promoter. Thus, due to selective binding of either AP-1 or C/EBP proteins, induction of GL e transcripts in mouse and human may be regulated differently. In addition to AP-1, NF-кB activity is also induced by CD40L stimulation in normal B cells. Using GST pulldown assays and coimmunoprecipitation techniques I show that NF-кB and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo . When Stat6 and NF-кB proteins are co-expressed in human embryonic kidney 293 (HEK 293) cells, an IL-4-inducible reporter gene containing both cognate binding sites in the promoter is synergistically activated in the presence of IL-4. Furthermore, the same IL-4-inducible reporter gene is also synergistically activated by the endogenous Stat6 and NF-кB proteins in IL-4-stimulated B lymphoma cells I.29μ. Consistently, by using nuclear extracts from transfected HEK 293 cells and from I.29μ B cells in electrophoretic mobility shift assays (EMSAs), I show that Stat6 and NF-кB bind cooperatively to a DNA probe containing both sites, and the presence of a complex formed by their cooperative binding correlates with the synergistic activation of the promoter by Stat6 and NF-кB. I conclude that the direct interaction between Stat6 and NF-кB may provide the basis for synergistic activation of the GL e promoter. Finally, although mouse GL e transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h and the promoter appears to be active for at least 2 days after B cell activation. These data suggest that induction of AP-1 and NF-кB activities by CD40L, although transient, is required for activation of the mouse GL e promoter by IL-4-induced Stat6." @default.
• W801142006 created "2016-06-24" @default.
• W801142006 creator A5018208196 @default.
• W801142006 date "2000-01-01" @default.
• W801142006 modified "2023-09-24" @default.
• W801142006 title "Regulation of Immunoglobulin Germline ε Transcripts by IL-4, CD40 Ligand and Lipopolysaccharide via Stat6, AP-1 and NF-кB Transcription Factors: A Dissertation" @default.
• W801142006 hasPublicationYear "2000" @default.
• W801142006 type Work @default.
• W801142006 sameAs 801142006 @default.
• W801142006 citedByCount "0" @default.
• W801142006 crossrefType "journal-article" @default.
• W801142006 hasAuthorship W801142006A5018208196 @default.
• W801142006 hasConcept C101762097 @default.
• W801142006 hasConcept C104317684 @default.
• W801142006 hasConcept C1292079 @default.
• W801142006 hasConcept C137756551 @default.
• W801142006 hasConcept C138885662 @default.
• W801142006 hasConcept C145430368 @default.
• W801142006 hasConcept C150194340 @default.
• W801142006 hasConcept C153911025 @default.
• W801142006 hasConcept C159654299 @default.
• W801142006 hasConcept C161733203 @default.
• W801142006 hasConcept C174510640 @default.
• W801142006 hasConcept C179926584 @default.
• W801142006 hasConcept C185592680 @default.
• W801142006 hasConcept C203014093 @default.
• W801142006 hasConcept C2778453870 @default.
• W801142006 hasConcept C2780115692 @default.
• W801142006 hasConcept C41895202 @default.
• W801142006 hasConcept C55493867 @default.
• W801142006 hasConcept C86339819 @default.
• W801142006 hasConcept C86803240 @default.
• W801142006 hasConcept C95444343 @default.
• W801142006 hasConceptScore W801142006C101762097 @default.
• W801142006 hasConceptScore W801142006C104317684 @default.
• W801142006 hasConceptScore W801142006C1292079 @default.
• W801142006 hasConceptScore W801142006C137756551 @default.
• W801142006 hasConceptScore W801142006C138885662 @default.
• W801142006 hasConceptScore W801142006C145430368 @default.
• W801142006 hasConceptScore W801142006C150194340 @default.
• W801142006 hasConceptScore W801142006C153911025 @default.
• W801142006 hasConceptScore W801142006C159654299 @default.
• W801142006 hasConceptScore W801142006C161733203 @default.
• W801142006 hasConceptScore W801142006C174510640 @default.
• W801142006 hasConceptScore W801142006C179926584 @default.
• W801142006 hasConceptScore W801142006C185592680 @default.
• W801142006 hasConceptScore W801142006C203014093 @default.
• W801142006 hasConceptScore W801142006C2778453870 @default.
• W801142006 hasConceptScore W801142006C2780115692 @default.
• W801142006 hasConceptScore W801142006C41895202 @default.
• W801142006 hasConceptScore W801142006C55493867 @default.
• W801142006 hasConceptScore W801142006C86339819 @default.
• W801142006 hasConceptScore W801142006C86803240 @default.
• W801142006 hasConceptScore W801142006C95444343 @default.
• W801142006 hasLocation W8011420061 @default.
• W801142006 hasOpenAccess W801142006 @default.
• W801142006 hasPrimaryLocation W8011420061 @default.
• W801142006 hasRelatedWork W1501402501 @default.
• W801142006 hasRelatedWork W1525781589 @default.
• W801142006 hasRelatedWork W1558954552 @default.
• W801142006 hasRelatedWork W1588231470 @default.
• W801142006 hasRelatedWork W1651302448 @default.
• W801142006 hasRelatedWork W1919937099 @default.
• W801142006 hasRelatedWork W1921504045 @default.
• W801142006 hasRelatedWork W1984593535 @default.
• W801142006 hasRelatedWork W1984879610 @default.
• W801142006 hasRelatedWork W2002883144 @default.
• W801142006 hasRelatedWork W2017718332 @default.
• W801142006 hasRelatedWork W2022518777 @default.
• W801142006 hasRelatedWork W2025447458 @default.
• W801142006 hasRelatedWork W2085309120 @default.
• W801142006 hasRelatedWork W2090254049 @default.
• W801142006 hasRelatedWork W2137016617 @default.
• W801142006 hasRelatedWork W2140729777 @default.
• W801142006 hasRelatedWork W2148859282 @default.
• W801142006 hasRelatedWork W2158938816 @default.
• W801142006 hasRelatedWork W2165886588 @default.
• W801142006 isParatext "false" @default.
• W801142006 isRetracted "false" @default.
• W801142006 magId "801142006" @default.
• W801142006 workType "article" @default.