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- W8022402 abstract "This chapter describes the application of negative staining and cryo-EM to the cytoskeleton research, highlighting the feasibility of correlating information on the living cell with electron microscopy for delivering meaningful structural information. Three major filament systems make up the cytoskeleton: actin filaments, microtubules, and intermediate filaments. When not in organized bundles, actin filaments are poorly preserved after dehydration and embedding in plastic. This fact, taken together with the open, three-dimensional nature of the cytoskeleton networks has limited the information obtainable by conventional EM methods based on thin sectioning. A freeze-plunging device, either home-made or a commercial apparatus housed in a chemical extractor hood, is needed. At the base of the plunger is a polystyrene box for liquid nitrogen and into this sits a small container for condensing ethane. Thin plastic films on grid supports, as used for conventional EM, can be used as a growth substrate for cultured cells. In order to render the film more stable in the electron beam, an additional layer of carbon deposited onto the grid can be used." @default.
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- W8022402 date "2006-01-01" @default.
- W8022402 modified "2023-09-26" @default.
- W8022402 title "Electron Microscopy of Extracted CytoskeletonsNegative Staining, Cryoelectron Microscopy, and Correlation with Light Microscopy" @default.
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- W8022402 doi "https://doi.org/10.1016/b978-012164730-8/50155-6" @default.
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