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- W80328168 abstract "Since evidence indicates that phorbol ester (PE)-induced production of Interleukin 2 requires transcription, the authors investigated the possibility that the PE receptor acts directly in the nuclei of EL4 thymoma cells. Using a procedure that maintained the integrity of the double nuclear membrane and minimized the plasma membrane (PM) contamination, they were unable to detect specific binding of (/sup 3/H)-phorbol 12,13-dibutyrate (/sup 3/H-PDB) in nuclei of unstimulated cells. Treatment of cells with 100 nM PDB for up to 6 hours did not cause appearance of PE binding capacity in nuclei despite the translocation of 40% of the cytosolic binding capacity to the plasma membrane fraction. The failure to detect nuclear binding capacity in treated cells was not due to occupation of nuclear sites with unlabeled ligand; effective exchange binding was demonstrated by recovery of total homogenate binding capacity in treated cells of 82 +/- 13% of that in untreated cells. Treatment of isolated nuclei with DNase to liberate DNA binding proteins also failed to reveal any nuclear PE binding capacity. Assay of nuclei for protein kinase C enzymatic activity gave similar negative results. These data argue strongly against a direct action of the intact PE receptor, or the PE bindingmore » fragment, in the transcriptional activation of Interleukin 2 in EL4 cells.« less" @default.
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- W80328168 date "1987-05-01" @default.
- W80328168 modified "2023-09-24" @default.
- W80328168 title "Lack of Phorbol ester binding in nuclei of EL-4 thymoma cells" @default.
- W80328168 hasPublicationYear "1987" @default.
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