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• W80459421 abstract "Article1 December 1985free access Photoregulated expression of a pea rbcS gene in leaves of transgenic plants F. Nagy F. Nagy Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author G. Morelli G. Morelli Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author R.T. Fraley R.T. Fraley Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA Search for more papers by this author S.G. Rogers S.G. Rogers Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA Search for more papers by this author N.-H. Chua N.-H. Chua Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author F. Nagy F. Nagy Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author G. Morelli G. Morelli Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author R.T. Fraley R.T. Fraley Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA Search for more papers by this author S.G. Rogers S.G. Rogers Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA Search for more papers by this author N.-H. Chua N.-H. Chua Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA Search for more papers by this author Author Information F. Nagy1, G. Morelli1, R.T. Fraley2, S.G. Rogers2 and N.-H. Chua1 1Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA 2Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA The EMBO Journal (1985)4:3063-3068https://doi.org/10.1002/j.1460-2075.1985.tb04046.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A 2.4-kb pea genomic fragment, containing a member (rbcS-E9) of the multigene family encoding the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase, was inserted into a non-oncogenic, Ti-plasmid vector and introduced into the genomes of Petunia hybrida (Mitchell) and Nicotiana tabacum (SR1) plants by in vitro transformation. Petunia and tobacco plants containing the introduced pea rbcS-E9 gene were regenerated from protoplasts. In these transgenic plants the rbcS-E9 gene is transcribed accurately using its own promoter and its expression is light-induced and organ-specific. A deletion mutant with 352 bp of 5′-upstream sequence still retains photoinducibility and leaf-specific expression. Clonal analysis of independent transgenic petunia plants revealed that chromosomal positions in the recipient plant genome affect the quantitative but not qualitative aspects of rbcS-E9 expression. Previous ArticleNext Article Volume 4Issue 121 December 1985In this issue RelatedDetailsLoading ..." @default.
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• W80459421 title "Photoregulated expression of a pea <i>rbc</i> S gene in leaves of transgenic plants" @default.
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