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- W805607688 abstract "There is an increasing need for development of laboratoryidentification procedures for purposes of plant variety protection, seed certification, quality control and consumer protection. In the past there were fewer commercial varieties and fieldtesting methods based on morphological differences were used satisfactorily. These tests require several months to complete and aresubsequently of limited practical use. With the large number ofcurrent varieties, visual differences are much smaller and thetask of identification is increasingly difficult.This study was initiated to investigate various morphological, chemical and biochemical characteristics of 25 varietiesgrown in the Pacific Northwest.The five tests described in this study provide the basis fora laboratory identification system for these varieties. The coleoptilecolor test was of limited value for these varieties, since onlyGolden exhibited a purple coleoptile while the remaining varietieswere green. The seedling pubescence test separated the varieties intotwo groups: slightly pubescent and pubescent. However, thevariation of the pubescence density caused by environmental conditions limits its practical use.The phenol test separated the varieties into three distinctgroups: Light Brown, Brown, and Mixed. Four varieties, Barbee,Daws, Druchamp, and Luke, exhibited a mixed phenol reaction. Thismixture was characteristic of these varieties since they were uniform for the other characters.Esterase, catalase, acid phosphatase and glutamic oxaloacetictransaminase were not useful for separating the varieties sinceall of them developed similar banding patterns for each enzyme.Peroxidase electrophoresis separated the varieties into two distinct groups on the basis of the presence or absence of a singleband.Gliadin electrophoresis was the most discriminating test,distinguishing all the varieties except for those geneticallyclosely related. These included varieties within three varietygroups: Gaines and Nugaines; Fielder and Fieldwin; and Hyslop,Hill, McDermid, and Stephens. The phenol test provided a usefulseparation of the group of four varieties which were uniform forgliadins. Hill and Hyslop developed a brown phenol stain whileMcDermid and Stephens exhibited a light brown stain. Furtheridentification might be possible by other means, such as pathogen resistance, isoelectric focusing and other chemical tests.In practice, unknown samples should be compared to authentic,standard samples for final identification." @default.
- W805607688 created "2016-06-24" @default.
- W805607688 creator A5069020511 @default.
- W805607688 date "1983-01-04" @default.
- W805607688 modified "2023-09-25" @default.
- W805607688 title "Laboratory methods for wheat variety identification" @default.
- W805607688 hasPublicationYear "1983" @default.
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