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• W806308631 abstract "Prior to this study, there has yet to be a clear demonstration of an artificial control of antibody affinity via photochromic dyes. The research described in this thesis sets out to address this by investigating photochromic dyes and their subsequent applications with high affinity biomolecules primarily to photomodulate the functions of biomolecules. The main avenue of investigation explored the conjugation of photochromic dyes (spiropyrans) to proteins (an enzyme and five different antibodies), to achieve reversible photomodulation of protein function for possible applications in biosensor technology (such as the development of reagentless bioreversible sensing systems). A secondary aim involved the investigation of the feasibility of antibody-antigen binding in the presence of ionic liquids. Ionic liquids have recently experienced growing interest as replacements for traditional organic solvents in a number of industrial applications. The practicability of spiropyrans in ionic liquids was also investigated (with the future possibility of photomodulated antibody-antigen interactions in ionic liquids to deliver a variety of improved analytical performances). The synthesis and photoswitching properties of an appropriate range of spiropyran dyes are reported. The spiropyran dyes are synthesised to possess a carboxyl group to aid carbodiimide mediated conjugation to lysine amino groups of proteins. The photochromic behaviour of the spiropyran dyes in various solvents, temperature and pH ranges were observed. Conjugation of carboxylated spiropyran dyes to an enzyme: horseradish peroxidise, was initially observed to aid development of experimental protocol for the target study group i.e. antibodies. Photomodulation of the modified horseradish peroxidase was found to demonstrate ~ 60 % decline in enzyme activity, an effect which was reversible as a result of the photoswitching capabilities of the attached spiropyran dyes. The five different antibodies; anti Atrazine, anti GroEL, anti Phytanic Acid, anti FITC and anti Staphylococcus aureus were modified with spiropyran dyes as with horseradish peroxidase. Reversible antibody affinity photomodulation was observed via their reaction in an ELISA which" @default.
• W806308631 created "2016-06-24" @default.
• W806308631 creator A5019865056 @default.
• W806308631 date "2008-09-01" @default.
• W806308631 modified "2023-09-27" @default.
• W806308631 title "Optical modulation of high-affinity biomolecules function via photochromic dyes : a step towards an artificial control of biological activity" @default.
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• W806308631 hasPublicationYear "2008" @default.
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