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• W807133990 abstract "ABSTRACT A strain of the filamentous fungus Aspergillus niger was isolated and shown to possess extracellular xylanolytic activity. These enzymes have biotechnological potential and can be employed in various industries. This fungus produced its highest xylanase activity in a medium made up of 0.1% CaCO 3 , 0.5% NaCl, 0.1% NH 4 Cl, 0.5% corn steep liquor and 1% carbon source, at pH 8.0. A low- cost hemicellulose residue (powdered corncob) proved to be an excellent inducer of the A. niger xylanolytic complex . Filtration of the crude culture medium with suspended kaolin was ideal for to clarify the extract and led to partial purification of the xylanolytic activity. The apparent molecular mass of the xylanase was about 32.3 kDa. Maximum enzyme activity occurred at pH 5.0 and 55-60oC. Apparent K m was 10.41 ± 0.282 mg/mL and V max was 3.32 ± 0.053 U/mg protein, with birchwood xylan as the substrate. Activation energy was 4.55 kcal/mol and half-life of the crude enzyme at 60oC was 30 minutes. Addition of 2% glucose to the culture medium supplemented with xylan repressed xylanase production, but in the presence of xylose the enzyme production was not affected. Keywords: Aspergillus niger.Xylanolytic enzymes.Thermostability. Agroindustrial residues RESUMO Fontes de carbono de baixo custo para a producao de xilanase termoestavel por Aspergillus niger Uma linhagem do fungo filamentoso Aspergillus niger foi isolada e apresentou atividade xilanolitica extracelular. Estas enzimas possuem grande potencial biotecnologico e podem ser aplicadas em diversas industrias. O fungo produziu sua maior atividade de xilanase em um meio contendo CaCO 3 0,1%, NaCl 0,5%, NH 4 Cl 0,1%, 0,5% agua de maceracao de milho e 1% de fonte de carbono, em pH 8,0. Um residuo lignocelulosico de baixo custo (sabugo de milho em po) mostrou ser um excelente indutor do complexo xilanolitico em A. niger . A filtracao do extrato cru com caulim foi ideal para a clarificacao do extrato e levou a purificacao parcial da enzima. A massa molecular aparente da xilanase foi de 32,3 kDa. A maxima atividade da enzima ocorreu em pH 5,0 e a 55-60oC. O K m aparente foi de 10,41 ± 0,282 mg/mL e a V max foi de 3,32 ± 0,053 U/mg proteina, utilizando-se xilana birchwood como substrato. A energia de ativacao foi de 4,55 kcal/mol, e a meia-vida da enzima a 60oC foi de 30 minutos. A adicao de 2% de glicose ao meio de cultura suplementado com xilana reprimiu a producao de xilanase, mas em presenca de xilose a producao da enzima nao foi afetada. Palavras-chave: Aspergillus niger.Enzimas xilanoliticas.Termoestabilidade. Residuos agroindustriais." @default.
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• W807133990 date "2013-08-04" @default.
• W807133990 modified "2023-10-03" @default.
• W807133990 title "Low-cost carbon sources for the production of a thermostable xylanase by Aspergillus niger" @default.
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