Matches in SemOpenAlex for { <https://semopenalex.org/work/W8076670> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W8076670 abstract "We tested the hypothesis that Superoxide (O2·−) mediates Ang II-dependent increase in expression of smooth muscle α-actin (SMα-actin) gene in cultured renal preglomerular microvascular smooth muscle cells (PGSMCs) from SHR. O2·− quantified by lucigenin method was significantly increased by 85% in intact PGSMCs and by 47% in cell membranes at 12 hr after incubation with Ang II (1uM). Expression of SMα-actin mRNA determined by real time RT-PCR was enhanced by 81% by Ang II and inhibited by 50% in Ang II-treated cells by the thiol antioxidant, N-acetyl-L-cysteine (NAC, 20mM) which also reduced Ang II-induced O2·− generation to 20%. Serum response factor (SRF) and its co-activator, myocardin (Myo) mediate CArG-dependent transcription of SMα-actin gene. Although Ang II treatment did not modify mRNA for SRF, it increased Myo expression by 22%. Promoter repoter assays indicated that Ang II increased SMα-actin promoter activity by 2.3 fold and this was inhibited by 43% by NAC. Site-directed mutations demonstrated that CArG elements are required for SMα-actin promoter activity. Quantitative chromatin immunoprecipitation assay revealed that Ang II increased SRF enrichment of CArG regions in SMα-actin promoter in intact chromatin by 57%, whereas NAC reduced it by 43% without affecting histone 3 (H3) acetylation or histone 3 lysine-9 (H3K9) dimethylation. In conclution; Ang II induces O2·− in SHR PGSMCs that increases SMα-actin gene expression via up-regulation of Myo and enhanced SRF bound to CArG elements without modifications of H3 acetylation and H3K9 methylation in the SMα-actin promoter regions." @default.
- W8076670 created "2016-06-24" @default.
- W8076670 creator A5009668864 @default.
- W8076670 creator A5011665458 @default.
- W8076670 creator A5013049990 @default.
- W8076670 creator A5041309784 @default.
- W8076670 creator A5043650697 @default.
- W8076670 creator A5051880293 @default.
- W8076670 creator A5075088009 @default.
- W8076670 creator A5083663683 @default.
- W8076670 date "2006-03-01" @default.
- W8076670 modified "2023-09-23" @default.
- W8076670 title "Angiotensin II induced reactive oxygen species modulate smooth muscle α‐actin gene expression via increased SRF binding to CArG cis elements in SHR renal microvascular smooth muscle cells" @default.
- W8076670 doi "https://doi.org/10.1096/fasebj.20.5.a1148-a" @default.
- W8076670 hasPublicationYear "2006" @default.
- W8076670 type Work @default.
- W8076670 sameAs 8076670 @default.
- W8076670 citedByCount "2" @default.
- W8076670 countsByYear W80766702019 @default.
- W8076670 countsByYear W80766702020 @default.
- W8076670 crossrefType "journal-article" @default.
- W8076670 hasAuthorship W8076670A5009668864 @default.
- W8076670 hasAuthorship W8076670A5011665458 @default.
- W8076670 hasAuthorship W8076670A5013049990 @default.
- W8076670 hasAuthorship W8076670A5041309784 @default.
- W8076670 hasAuthorship W8076670A5043650697 @default.
- W8076670 hasAuthorship W8076670A5051880293 @default.
- W8076670 hasAuthorship W8076670A5075088009 @default.
- W8076670 hasAuthorship W8076670A5083663683 @default.
- W8076670 hasConcept C101762097 @default.
- W8076670 hasConcept C104317684 @default.
- W8076670 hasConcept C125705527 @default.
- W8076670 hasConcept C134320426 @default.
- W8076670 hasConcept C150194340 @default.
- W8076670 hasConcept C153911025 @default.
- W8076670 hasConcept C170493617 @default.
- W8076670 hasConcept C185592680 @default.
- W8076670 hasConcept C2908929049 @default.
- W8076670 hasConcept C48349386 @default.
- W8076670 hasConcept C55493867 @default.
- W8076670 hasConcept C64927066 @default.
- W8076670 hasConcept C69053785 @default.
- W8076670 hasConcept C86803240 @default.
- W8076670 hasConcept C88045685 @default.
- W8076670 hasConcept C95444343 @default.
- W8076670 hasConceptScore W8076670C101762097 @default.
- W8076670 hasConceptScore W8076670C104317684 @default.
- W8076670 hasConceptScore W8076670C125705527 @default.
- W8076670 hasConceptScore W8076670C134320426 @default.
- W8076670 hasConceptScore W8076670C150194340 @default.
- W8076670 hasConceptScore W8076670C153911025 @default.
- W8076670 hasConceptScore W8076670C170493617 @default.
- W8076670 hasConceptScore W8076670C185592680 @default.
- W8076670 hasConceptScore W8076670C2908929049 @default.
- W8076670 hasConceptScore W8076670C48349386 @default.
- W8076670 hasConceptScore W8076670C55493867 @default.
- W8076670 hasConceptScore W8076670C64927066 @default.
- W8076670 hasConceptScore W8076670C69053785 @default.
- W8076670 hasConceptScore W8076670C86803240 @default.
- W8076670 hasConceptScore W8076670C88045685 @default.
- W8076670 hasConceptScore W8076670C95444343 @default.
- W8076670 hasIssue "5" @default.
- W8076670 hasLocation W80766701 @default.
- W8076670 hasOpenAccess W8076670 @default.
- W8076670 hasPrimaryLocation W80766701 @default.
- W8076670 hasRelatedWork W1518531052 @default.
- W8076670 hasRelatedWork W1547599546 @default.
- W8076670 hasRelatedWork W2004766837 @default.
- W8076670 hasRelatedWork W2008509160 @default.
- W8076670 hasRelatedWork W2015958124 @default.
- W8076670 hasRelatedWork W2104961127 @default.
- W8076670 hasRelatedWork W2136768622 @default.
- W8076670 hasRelatedWork W2167746653 @default.
- W8076670 hasRelatedWork W236146588 @default.
- W8076670 hasRelatedWork W2981075320 @default.
- W8076670 hasVolume "20" @default.
- W8076670 isParatext "false" @default.
- W8076670 isRetracted "false" @default.
- W8076670 magId "8076670" @default.
- W8076670 workType "article" @default.