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- W809957075 abstract "Two Rhizobium etli Tn5mob -induced mutants (CFN035 and CFN037) exhibited enhanced capacity to oxidize N,N,N′,N′,tetramethyl-p -phenylenediamine (TMPD), a presumptive indicator of elevated cytochrome c terminal oxidase. Sequence of the mutated gene in CFN035 revealed that it codes for the amidophosphoribosyl-transferase enzyme (purF), catalyzing the first step of the purine biosynthetic pathway (1). In CFN037 the Tn5mob insertion was located in the promoter region of thethiCOGE gene cluster and promotes a constitutive expression of thiC (thiC C mutant). 4-methyl-5-(s-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethylpyrimidine pyrophosphate (HMP-P), are coupled to form thiamin monophosphate, which is phosphorylated to make thiamin pyrophosphate. ThiC from R. etli shared significant homology with thiC from E. coli which is involved in the synthesis of HMP from the purine intermediate 5-Aminoimidazole-ribonucleotide. The second ORF of 327 residues is the product of a novel gene which is denoted as thiO. Analysis of the protein sequence suggests that ThiO catalyzes the oxidative deamination of some intermediate of thiamin biosynthesis. ThiG and ThiE from R. etli shared significant homology with ThiG and thiE from E. coli which are involved in the synthesis of THZ and in the condensation of HMP-P with THZ-P respectively. CFN035 and CFN037 produced the cbb 3 terminal oxidase as did the wild-type R. etli strain expressing the B. japonicum fixNOQP genes, which code for the symbiotic cbb 3 terminal oxidase. A blockade in the first step of the purine biosynthetic pathway and the constitutive expression of thiC would lower the concentration of several metabolites of the purine biosynthetic pathway. In order to identify the possible metabolic effector involved in cbb 3, production, the expression of a R. etli fixN-lacZ gene fusion was measured in several mutants affected in different steps of the purine biosynthetic pathway." @default.
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- W809957075 date "1998-01-01" @default.
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- W809957075 title "Characterization of R. etli Mutants in the Purine-Thiamin Metabolism Suggest That 5-Aminoimidazole-4-Carboxamide Ribonucleotide (AICAR) is a Negative Effector of Symbiotic Cytochrome Terminal Oxidase cbb3 Production" @default.
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- W809957075 doi "https://doi.org/10.1007/978-94-011-5159-7_60" @default.
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