Matches in SemOpenAlex for { <https://semopenalex.org/work/W81285192> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W81285192 endingPage "1245" @default.
- W81285192 startingPage "1241" @default.
- W81285192 abstract "AIM: TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) has been reported to specifically induce apoptosis of cancer cells although only a small percentage of cell lines were sensitive to it. Cell lines not responding to TRAIL in vitro were said to be more prone to apoptosis when TRAIL was combined with another anticancer agent.Generally, factors affecting drug-sensitivity involve many apoptosis-related proteins, including p53. The expression of wild-type p53 gene was proposed as an important premise for tumor cells responding to chemotherapy. The present study was to investigate the cell killing action of TRAIL on colon cancer cell line SW480, its synergistic effect with doxorubicin, and the possible mechanisms.METHODS: SW480 cells were cultured in the regular condition and incubated with different levels of agents.Morphologic changes in these cells after treatment were observed under phase-contrast microscope and cytotoxicity by TRAIL alone and in combination with doxorubicin was quantified by a 1-day microculture tetrazolium dye (MTT) assay. In addition, flow cytometry assay (FCM) and transmission electron microscopy were used to detect apoptosis among these cells. Variation of p53 protein level among different groups according to concentrations of agents was measured by Western blot assay.RESULTS: (1) SW480 cells were not sensitive to TRAIL,with IC50>l mg·L^1 and dose-independent cytotoxicity. (2)SW480 cells were sensitive to doxorubicin at a certain degree,with dose-dependent cytotoxicity and IC50=65.25±3.48μmol·L^-1. (3) TRAIL could synergize with doxorubicin to kill SW480 cells effectively, which was represented by the boosted killing effect of doxorubicin on theses cells. IC50 of doxorubicin against SW480 cells sharply reduced when it was combined with TRAIL. (4) Subtoxic TRAIL (100 μg·L^-1),combined with subtoxic doxorubicin (0.86 μmol·L^-1), could kill SW480 cells sufficiently. Cytotoxicity by MTT assay arrived at 80.12±2.67 %, which was significantly higher than that by TRAIL or d" @default.
- W81285192 created "2016-06-24" @default.
- W81285192 creator A5001119812 @default.
- W81285192 creator A5002458492 @default.
- W81285192 creator A5003303388 @default.
- W81285192 creator A5054655763 @default.
- W81285192 creator A5055669910 @default.
- W81285192 date "2003-01-01" @default.
- W81285192 modified "2023-09-24" @default.
- W81285192 title "Synergistic antitumor effect of TRAIL and doxorubicin on colon cancer cell line SW480" @default.
- W81285192 hasPublicationYear "2003" @default.
- W81285192 type Work @default.
- W81285192 sameAs 81285192 @default.
- W81285192 citedByCount "0" @default.
- W81285192 crossrefType "journal-article" @default.
- W81285192 hasAuthorship W81285192A5001119812 @default.
- W81285192 hasAuthorship W81285192A5002458492 @default.
- W81285192 hasAuthorship W81285192A5003303388 @default.
- W81285192 hasAuthorship W81285192A5054655763 @default.
- W81285192 hasAuthorship W81285192A5055669910 @default.
- W81285192 hasConcept C109316439 @default.
- W81285192 hasConcept C121608353 @default.
- W81285192 hasConcept C153911025 @default.
- W81285192 hasConcept C17991360 @default.
- W81285192 hasConcept C185592680 @default.
- W81285192 hasConcept C190283241 @default.
- W81285192 hasConcept C202751555 @default.
- W81285192 hasConcept C203014093 @default.
- W81285192 hasConcept C2776694085 @default.
- W81285192 hasConcept C2780783641 @default.
- W81285192 hasConcept C2781303535 @default.
- W81285192 hasConcept C29537977 @default.
- W81285192 hasConcept C502942594 @default.
- W81285192 hasConcept C54355233 @default.
- W81285192 hasConcept C553184892 @default.
- W81285192 hasConcept C55493867 @default.
- W81285192 hasConcept C81885089 @default.
- W81285192 hasConcept C86803240 @default.
- W81285192 hasConcept C96232424 @default.
- W81285192 hasConcept C98274493 @default.
- W81285192 hasConceptScore W81285192C109316439 @default.
- W81285192 hasConceptScore W81285192C121608353 @default.
- W81285192 hasConceptScore W81285192C153911025 @default.
- W81285192 hasConceptScore W81285192C17991360 @default.
- W81285192 hasConceptScore W81285192C185592680 @default.
- W81285192 hasConceptScore W81285192C190283241 @default.
- W81285192 hasConceptScore W81285192C202751555 @default.
- W81285192 hasConceptScore W81285192C203014093 @default.
- W81285192 hasConceptScore W81285192C2776694085 @default.
- W81285192 hasConceptScore W81285192C2780783641 @default.
- W81285192 hasConceptScore W81285192C2781303535 @default.
- W81285192 hasConceptScore W81285192C29537977 @default.
- W81285192 hasConceptScore W81285192C502942594 @default.
- W81285192 hasConceptScore W81285192C54355233 @default.
- W81285192 hasConceptScore W81285192C553184892 @default.
- W81285192 hasConceptScore W81285192C55493867 @default.
- W81285192 hasConceptScore W81285192C81885089 @default.
- W81285192 hasConceptScore W81285192C86803240 @default.
- W81285192 hasConceptScore W81285192C96232424 @default.
- W81285192 hasConceptScore W81285192C98274493 @default.
- W81285192 hasIssue "6" @default.
- W81285192 hasLocation W812851921 @default.
- W81285192 hasOpenAccess W81285192 @default.
- W81285192 hasPrimaryLocation W812851921 @default.
- W81285192 hasRelatedWork W2150008477 @default.
- W81285192 hasRelatedWork W2350266416 @default.
- W81285192 hasRelatedWork W2351248321 @default.
- W81285192 hasRelatedWork W2351574517 @default.
- W81285192 hasRelatedWork W2353993958 @default.
- W81285192 hasRelatedWork W2354509341 @default.
- W81285192 hasRelatedWork W2355828621 @default.
- W81285192 hasRelatedWork W2358929219 @default.
- W81285192 hasRelatedWork W2360785416 @default.
- W81285192 hasRelatedWork W2363247631 @default.
- W81285192 hasRelatedWork W2370329723 @default.
- W81285192 hasRelatedWork W2375224626 @default.
- W81285192 hasRelatedWork W2380413443 @default.
- W81285192 hasRelatedWork W2387195251 @default.
- W81285192 hasRelatedWork W2388527977 @default.
- W81285192 hasRelatedWork W2389756890 @default.
- W81285192 hasRelatedWork W2403138433 @default.
- W81285192 hasRelatedWork W2421574970 @default.
- W81285192 hasRelatedWork W3032241117 @default.
- W81285192 hasRelatedWork W3148473862 @default.
- W81285192 hasVolume "9" @default.
- W81285192 isParatext "false" @default.
- W81285192 isRetracted "false" @default.
- W81285192 magId "81285192" @default.
- W81285192 workType "article" @default.