Matches in SemOpenAlex for { <https://semopenalex.org/work/W81492632> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W81492632 abstract "We have shown that HKa as well as domain (D5) inhibit migration, proliferation and induce apoptosis in vitro. To study its effect on tube formation we utilized a collagen-fibrinogen, three-dimensional gel, a model of angiogenesis. Collagen and fibrinogen (1:2) were mixed with endothelial cells and an angiogenic stimulator (a mixture of bFGF, VEGF and PMA) was added to the gel in order to induce the formation of capillary structures. The anti-angiogenic effect of HKa, GST-D5 and D5 as well as peptides (300 nM) on tube formation was evaluated by measuring tube length. HKa, GST-D5 and D5 inhibit tube formation by 90%, 86%, 77% respectively. Peptides G486-K502, H475-H485 and G440-H455 inhibited by 77%, 54%, 51%, respectively. In a two dimensional cell culture system, HKa can detach endothelial cells from dishes coated with fibrinogen which is a ligand of integrin αvβ3 and α5β1. HKa can block the formation of the complex of uPAR and integrin α5β1 from 1 to 26 hours as shown by immunoprecipitation studies. Using SiRNA, we down-regulated C-terminal Src kinase expression by 80% and significantly increased tube length by 27%. The addition of HKa almost completely blocked this effect. We further found that PP2, a Src inhibitor, dose-dependently blocked tube formation in 3D gel. Since we have previously shown that uPAR can form a complex with integrins, caveolin and Src kinase, it is likely that HKa as well as D5 bound to uPAR and disrupted this complex. This outcome would further downregulate the Src kinase activity which in turn could inhibit tube formation in the 3D gel." @default.
- W81492632 created "2016-06-24" @default.
- W81492632 creator A5006468559 @default.
- W81492632 creator A5051377664 @default.
- W81492632 creator A5069143752 @default.
- W81492632 date "2006-03-01" @default.
- W81492632 modified "2023-09-25" @default.
- W81492632 title "Cleaved kininogen (HKa) inhibits tube formation by endothelial cells in a collagen‐fibrinogen, three‐dimensional gel and is dependent on Src kinase" @default.
- W81492632 doi "https://doi.org/10.1096/fasebj.20.5.a925-b" @default.
- W81492632 hasPublicationYear "2006" @default.
- W81492632 type Work @default.
- W81492632 sameAs 81492632 @default.
- W81492632 citedByCount "0" @default.
- W81492632 crossrefType "journal-article" @default.
- W81492632 hasAuthorship W81492632A5006468559 @default.
- W81492632 hasAuthorship W81492632A5051377664 @default.
- W81492632 hasAuthorship W81492632A5069143752 @default.
- W81492632 hasConcept C108636557 @default.
- W81492632 hasConcept C153911025 @default.
- W81492632 hasConcept C170493617 @default.
- W81492632 hasConcept C184235292 @default.
- W81492632 hasConcept C185592680 @default.
- W81492632 hasConcept C195687474 @default.
- W81492632 hasConcept C2777902427 @default.
- W81492632 hasConcept C2779036427 @default.
- W81492632 hasConcept C2779591629 @default.
- W81492632 hasConcept C2780394083 @default.
- W81492632 hasConcept C2781317560 @default.
- W81492632 hasConcept C502942594 @default.
- W81492632 hasConcept C55493867 @default.
- W81492632 hasConcept C56219504 @default.
- W81492632 hasConcept C86803240 @default.
- W81492632 hasConcept C95444343 @default.
- W81492632 hasConceptScore W81492632C108636557 @default.
- W81492632 hasConceptScore W81492632C153911025 @default.
- W81492632 hasConceptScore W81492632C170493617 @default.
- W81492632 hasConceptScore W81492632C184235292 @default.
- W81492632 hasConceptScore W81492632C185592680 @default.
- W81492632 hasConceptScore W81492632C195687474 @default.
- W81492632 hasConceptScore W81492632C2777902427 @default.
- W81492632 hasConceptScore W81492632C2779036427 @default.
- W81492632 hasConceptScore W81492632C2779591629 @default.
- W81492632 hasConceptScore W81492632C2780394083 @default.
- W81492632 hasConceptScore W81492632C2781317560 @default.
- W81492632 hasConceptScore W81492632C502942594 @default.
- W81492632 hasConceptScore W81492632C55493867 @default.
- W81492632 hasConceptScore W81492632C56219504 @default.
- W81492632 hasConceptScore W81492632C86803240 @default.
- W81492632 hasConceptScore W81492632C95444343 @default.
- W81492632 hasIssue "5" @default.
- W81492632 hasLocation W814926321 @default.
- W81492632 hasOpenAccess W81492632 @default.
- W81492632 hasPrimaryLocation W814926321 @default.
- W81492632 hasRelatedWork W1966682053 @default.
- W81492632 hasRelatedWork W1977093166 @default.
- W81492632 hasRelatedWork W2024406862 @default.
- W81492632 hasRelatedWork W2027346283 @default.
- W81492632 hasRelatedWork W2276184075 @default.
- W81492632 hasRelatedWork W2324850253 @default.
- W81492632 hasRelatedWork W2579168846 @default.
- W81492632 hasRelatedWork W4214558002 @default.
- W81492632 hasRelatedWork W4313380908 @default.
- W81492632 hasRelatedWork W81492632 @default.
- W81492632 hasVolume "20" @default.
- W81492632 isParatext "false" @default.
- W81492632 isRetracted "false" @default.
- W81492632 magId "81492632" @default.
- W81492632 workType "article" @default.