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• W815419215 abstract "With the increasing use of biodiesel in the world, there is great availability of glycerol in the market. In Brazil, it is expected a availability of 200 thousand tons of biodiesel glycerol with the mixture of 5% biodiesel into mineral biodiesel. Nevertheless, the purification and clarification of glycerol is a very costly process, and the use of raw glycerol to generate products with high added value, may reduce the price of biodiesel in the market, since raw glycerol storing and disposing costs are put on the biodiesel price. Raw glycerol is preferably used to produce 1,3 propanediol by bacteria, but little has been studied about its fermentation by yeasts, which can use larger amounts of glycerol and have higher conversion rates. Fermentations were performed with 30 yeast strains able to use glycerol as the sole carbon source using Erlenmeyer flask. The fermented broth was analyzed on HPLC equipped with UV and RI detector searching ethanol, propanol, butanol, lactate, propionate, citrate, succinate and acetate, 1,3 propanediol, 1,2 propanediol, and 2,3 butanediol. The method of experimental design was used for growth optimization and three fermentation were performed in fermentor vase to evaluate the optimized medium. Two experimental designs were made for the evaluate the production medium and six fermentation in fermentor vase varying aeration and agitation. It was found that Yarrowia lipolytica NRRL Y-1095 strain was the most promising in the use of glycerol to produce citrate. The growth medium was optimized in glycerol 50.0 g.L, yeast extract 5.0 gL, ammonium sulfate 1.0 gL, KH2PO4 7.0 gL , Na2HPO4 2.5 gL , FeCl3 0.65 mg.L , ZnSO4 1.2 mg.L , CuSO4.5H2O 0.31 mg.L -1 and MnSO4 0.27 mg.L , reaching 6.32 gL of dry weight in bottles and 23gL in fermentation reactor. It was found that glycerol was the only significant factor in the production of citrate and isocitrate using an experimental design varying glycerol and MgSO4. Also was observed that the dissolved oxygen was a important factor, and using fermentation reactor with 1.0 vvm of aeration and 800 rpm, was obtained 59 g L citric acid." @default.
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• W815419215 date "2011-03-17" @default.
• W815419215 modified "2023-09-30" @default.
• W815419215 title "Prospecção de bioprodutos de interesse industrial a partir da fermentação do glicerol por leveduras" @default.
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• W815419215 doi "https://doi.org/10.5016/dt000618833" @default.
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