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- W81567862 abstract "Mass spectrometry (MS) in combination with chromatographic separation techniques such as liquid chromatography (LC) and gas chromatography (GC) is widely used in metabolomics. Shortcomings include the lack of comprehensive mass spectral libraries for LC-MS, the frequent need for derivatization in GC-MS, and the lack of metabolite mass spectral libraries for derivatives. The lack of comprehensive mass spectral libraries can be overcome using soft ionization techniques. In combination with high-resolution MS, measurement of accurate mass of the quasi-molecular ion allows elemental formulas to be searched against metabolite databases. This chapter describes the basics of LC, GC and MS and discusses limitations in their application to metabolomics, with an emphasis on the identification of unknown analytes." @default.
- W81567862 created "2016-06-24" @default.
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- W81567862 date "2013-01-01" @default.
- W81567862 modified "2023-09-23" @default.
- W81567862 title "Equipment and metabolite identification (ID) strategies for mass-based metabolomic analysis" @default.
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- W81567862 doi "https://doi.org/10.1533/9780857098818.1.3" @default.
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