FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W815830710> ?p ?o ?g. }

• W815830710 abstract "Aims and scopeThis thesis aims to provide a basis for the microbial protein enrichment of sago starch by solid-state cultivation (SSC), and the design principles of packed bed solid-state cultivation systems. The development of design principles for solid-state biorectors has been hampered by the complexity of solid-state cultivation and the difficulty in performing experimental measurements of key process variables. In this thesis three systems of packed bed solid-state cultivation were developed, for the study of key parameters including aeration, substrate loading, particle size, homogeneity of the culture and drying of the microbial product.This thesis concentrates on a single organism (the filamentous fungus Rhizopus oligosporus UQM 145F) grown on a spherical sago-bead substrate, with an approach designed for more general applicability.Results and conclusionsThe work was carried out in five steps:(1) Choice of microorganism: Rhizopus oligosporus UQM 145F had greater potential for solid-state cultivations of sago starch than the other fungus considered, Rhizopus sp. UQM 186F. Without forced aeration in the flask culture R. oligosporus UQM 145F was able to produce 7.7 % protein (w/w dry basis) compared to only 4.9 % protein (w/w dry basis) by Rhizopus sp. UQM 186F in 60 h. The microbial protein content attained by R. oligosporus UQM 145F with forced aeration in the Sartorius packed bed bioreactor was 10.7 % (w/w dry basis) compared to 9.9 % (w/w dry basis) by Rhizopus sp. UQM 186F. The mycelium of R. oligosporus UQM 145F was strongly bound to the substrate, therefore not easily damaged by high air flowrates, making it suitable for the scaling-up studies in the bigger bioreactor.(2) Performing of basic studies for the growth improvement of R. oligosporus UQM 145F on sago-beads: This included the effects of Hortico trace element fertilizer and the concentration of nitrogenous compounds. A model substrate (Mitchell et al., 1986) was also used, since it was difficult to obtain a homogeneous sample for the study of C/N ratio and initial starch concentrations with the sago-beads. This work also included comparisons of methods for determination of the biomass protein content, and determination of the amino acid composition of the biomass.(3) Scaling-up studies performed in the standing column packed bed SSC system: This system was designed as an improvement of the Sartorius packed bed SSC system. The system was originally intended to be used for the study of SSC’s in both the expanded, and the packed bed regions. However, due to the natural sticky characteristics of the sago-beads, only studies in the packed bed region could be performed.During the studies it was found that the best air flowrate employed was an air flowrate of 12.5 l/min. The bioreactor was used for SSC’s with substrate loadings of 100 to 600 g. However, for the measurement of pressure drop across the bed, only substrate loadings of 200 to 350 g were used. The standing column packed bed SSC system also allowed drying of the microbial product in situ.(4) Investigation of the change in particle diameter of the sago-beads performed in the multiple mini packed bed SSC system: This system was designed to overcome the problems of heterogeneous conditions and non-representative samples which occurred in the Sartorius and the standing column packed bed SSC systems. The data of particle diameter, together with the data of pressure drop and bed height (obtained in the cultivations using the standing column bioreactor) were used to calculate the change in bed voidage using the Ergun equation, and the apparent fungal packing density during SSC in the standing column packed bed bioreactor.(5) Estimation of microbial protein production, during cultivations without removal of samples, using a correlation model. The model was adapted from the fungal density model developed by Laukevics et al. (1985), and the bed voidage model described by Auria et al. (1991). However, the correlation model can only be used at the period 30 to 54 h of the cultivation, the period where fungal biomass development has significantly filled in the interparticle void spaces.The approach used in this thesis can lead to significant advances in understanding fungal growth on substrate surfaces and in interparticle void spaces. The achievements arose from use of spherical sago-beads, and correlation of pressure drop across the bed with the change in bed voidage." @default.
• W815830710 created "2016-06-24" @default.
• W815830710 creator A5075976416 @default.
• W815830710 date "2014-11-24" @default.
• W815830710 modified "2023-09-27" @default.
• W815830710 title "Development of packed bed solid-state cultivation systems for the protein enrichment of sago starch" @default.
• W815830710 doi "https://doi.org/10.14264/uql.2014.416" @default.
• W815830710 hasPublicationYear "2014" @default.
• W815830710 type Work @default.
• W815830710 sameAs 815830710 @default.
• W815830710 citedByCount "0" @default.
• W815830710 crossrefType "dissertation" @default.
• W815830710 hasAuthorship W815830710A5075976416 @default.
• W815830710 hasConcept C100544194 @default.
• W815830710 hasConcept C133479454 @default.
• W815830710 hasConcept C178790620 @default.
• W815830710 hasConcept C185592680 @default.
• W815830710 hasConcept C2778753027 @default.
• W815830710 hasConcept C2779516289 @default.
• W815830710 hasConcept C2779942233 @default.
• W815830710 hasConcept C31903555 @default.
• W815830710 hasConcept C529335014 @default.
• W815830710 hasConcept C59822182 @default.
• W815830710 hasConcept C86803240 @default.
• W815830710 hasConcept C86922832 @default.
• W815830710 hasConceptScore W815830710C100544194 @default.
• W815830710 hasConceptScore W815830710C133479454 @default.
• W815830710 hasConceptScore W815830710C178790620 @default.
• W815830710 hasConceptScore W815830710C185592680 @default.
• W815830710 hasConceptScore W815830710C2778753027 @default.
• W815830710 hasConceptScore W815830710C2779516289 @default.
• W815830710 hasConceptScore W815830710C2779942233 @default.
• W815830710 hasConceptScore W815830710C31903555 @default.
• W815830710 hasConceptScore W815830710C529335014 @default.
• W815830710 hasConceptScore W815830710C59822182 @default.
• W815830710 hasConceptScore W815830710C86803240 @default.
• W815830710 hasConceptScore W815830710C86922832 @default.
• W815830710 hasLocation W8158307101 @default.
• W815830710 hasOpenAccess W815830710 @default.
• W815830710 hasPrimaryLocation W8158307101 @default.
• W815830710 hasRelatedWork W1964957709 @default.
• W815830710 hasRelatedWork W1984588357 @default.
• W815830710 hasRelatedWork W1987847234 @default.
• W815830710 hasRelatedWork W2046492420 @default.
• W815830710 hasRelatedWork W2048156048 @default.
• W815830710 hasRelatedWork W2065511458 @default.
• W815830710 hasRelatedWork W2072522914 @default.
• W815830710 hasRelatedWork W2076398543 @default.
• W815830710 hasRelatedWork W2080250942 @default.
• W815830710 hasRelatedWork W2093116827 @default.
• W815830710 hasRelatedWork W2098575077 @default.
• W815830710 hasRelatedWork W2114060617 @default.
• W815830710 hasRelatedWork W2124025542 @default.
• W815830710 hasRelatedWork W2168435689 @default.
• W815830710 hasRelatedWork W2520264243 @default.
• W815830710 hasRelatedWork W2534066989 @default.
• W815830710 hasRelatedWork W2582747849 @default.
• W815830710 hasRelatedWork W2622856957 @default.
• W815830710 hasRelatedWork W297681897 @default.
• W815830710 hasRelatedWork W3048901584 @default.
• W815830710 isParatext "false" @default.
• W815830710 isRetracted "false" @default.
• W815830710 magId "815830710" @default.
• W815830710 workType "dissertation" @default.